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  • KD/KO Validated

COX16 Polyclonal antibody

COX16 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 19425-1-AP

Synonyms

C14orf112, COX16, HSPC203, PTD019



Tested Applications

Positive WB detected inA549 cells, HeLa cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19425-1-AP targets COX16 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COX16 fusion protein Ag13756
Full Name COX16 cytochrome c oxidase assembly homolog (S. cerevisiae)
Calculated Molecular Weight 106 aa, 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC001702
Gene Symbol COX16
Gene ID (NCBI) 51241
RRIDAB_10666854
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for COX16 antibody 19425-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machinery

Authors - Jasmin Adriana Schafer
humanWB

Nat Commun

Coordination of metal center biogenesis in human cytochrome c oxidase.

Authors - Eva Nývltová
humanWB

Elife

COX16 promotes COX2 metallation and assembly during respiratory complex IV biogenesis.

Authors - Abhishek Aich
  • KO Validated
humanWB

J Biol Chem

An animal model for mitochondrial tyrosyl-tRNA synthetase deficiency reveals links between oxidative phosphorylation and retinal function.

Authors - Xiaofen Jin
mouseWB

Redox Biol

Lead aggravates Alzheimer's disease pathology via mitochondrial copper accumulation regulated by COX17

Authors - Dingbang Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Süleyman (Verified Customer) (02-07-2022)

There are some unspecific bands which were distant but stronger than COX16 itself, even though I loaded 30-40ug of protein and 1.1000 dilution of antibody, either HeLa cells have very low abundant or antibody has some problems.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa
COX16 Antibody Western Blot validation (1:1000 dilution) in HeLa (Cat no:19425-1-AP)