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Tested Applications
| Positive WB detected in | MCF-7 cells, rat heart tissue, THP-1 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 15 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
| CoIP | See 1 publications below |
Product Information
11464-1-AP targets COX17 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2032 Product name: Recombinant human COX17 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-63 aa of BC010933 Sequence: MPGLVDSNPAPPESQEKKPLKPCCACPETKKARDACIIEKGEEHCGHLIEAHKECMRALGFKI Predict reactive species |
| Full Name | COX17 cytochrome c oxidase assembly homolog (S. cerevisiae) |
| Calculated Molecular Weight | 63 aa, 7 kDa |
| Observed Molecular Weight | 7 kDa |
| GenBank Accession Number | BC010933 |
| Gene Symbol | COX17 |
| Gene ID (NCBI) | 10063 |
| RRID | AB_2085109 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14061 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for COX17 antibody 11464-1-AP | Download protocol |
| IHC protocol for COX17 antibody 11464-1-AP | Download protocol |
| WB protocol for COX17 antibody 11464-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.
| ||
Am J Hum Genet The mitochondrial disulfide relay system protein GFER is mutated in autosomal-recessive myopathy with cataract and combined respiratory-chain deficiency. | ||
Nutrients Diosgenin Inhibits ROS Generation by Modulating NOX4 and Mitochondrial Respiratory Chain and Suppresses Apoptosis in Diabetic Nephropathy | ||
FASEB J Exercise improves angiogenic function of circulating exosomes in type 2 diabetes: Role of exosomal SOD3. | ||
J Biol Chem An animal model for mitochondrial tyrosyl-tRNA synthetase deficiency reveals links between oxidative phosphorylation and retinal function. | ||
Toxicol Appl Pharmacol Copper accumulation and compartmentalization in mouse fibroblast lacking metallothionein and copper chaperone, Atox1. |
