COXIV Antibody
Rabbit Polyclonal
Catalog number: 11242-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF, IP |
| Cited applications | WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human, mouse, rat |
| Positive WB detected in | MCF7 cells, HEK-293 cells, A375 cells, A431 cells, A549 cells, HeLa cells, HepG2 cells, human brain tissue, human lung tissue, human skeletal muscle tissue, human skin tissue, human spleen tissue, Jurkat cells, mouse brain tissue, mouse heart tissue, mouse liver tissue, mouse skeletal muscle tissue, NIH/3T3 cells, L02 cells |
| Positive IHC detected in | human prostate cancer |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:50-1:200 IF: 1:20-1:100 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
IP result of COX4I1 antibody (11242-1-AP for IP and Detection) with mouse skeletal muscle tissue lysate..
MCF7 cells were subjected to SDS PAGE followed by western blot with 11242-1-AP(COXIV Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human prostate cancer using 11242-1-AP(COXIV Antibody) at Dilution 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using COX4I1 antibody 11242-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | COXIV fusion protein ag1640 | Full name | cytochrome c oxidase subunit IV isoform 1 |
|
Calculated molecular weight |
19.6kd |
Observed molecular
weight |
17-18kd |
|
GenBank accession number |
BC021236 | Gene ID (NCBI) | 1327 |
| Gene symbol | COX4I1 | Synonyms | COX4; COXIV; MGC72016 |
Background
COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody was generated against full length COX4I1 protein and cross reacts with COX4I2. This antibody is specific to COXIV. |
