COX4I2 AntibodyRabbit Polyclonal
|Tested applications||ELISA, WB, IHC, IF, IP|
|Species specificity||human, mouse, rat; other species not tested.|
|Cited species||human, rat|
|Positive WB detected in||MCF7 cells, A375 cells, HepG2 cells, human brain tissue, human heart tissue, human lung tissue|
|Positive IHC detected in||human ovary tumor|
|Positive IF detected in||Hela cells|
|Recommended dilution||WB: 1:500-1:5000|
IP = Immunoprecipitation
- IP result of anti-COX4I2 (11463-1-AP for IP and Detection) with HepG2 cell lysate.
- MCF7 cells were subjected to SDS PAGE followed by western blot with 11463-1-AP(COX4I2 Antibody) at dilution of 1:1000
- Immunohistochemistry of paraffin-embedded human ovary tumor using 11463-1-AP(COX4I2 Antibody) at Dilution 1:50 (under 10x lens)
- Immunofluorescent analysis of Hela cells, using COX4I2 antibody 11463-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
|Source||Rabbit||Purification method||Antigen affinity purification|
|Isotype||IgG||Storage||PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.|
|Immunogen||COX4I2 fusion protein ag1987||Full name||cytochrome c oxidase subunit IV isoform 2 (lung)|
|BC057779||Gene ID (NCBI)||84701|
|Gene symbol||COX4I2||Synonyms||COX4; COX4-2; COX4B; COX4L2; COXIV-2; dJ857M17.2|
COX4I2, also named as COX4L2 and COXIV-2, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COXIV(cytochrome c oxidase IV) has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. This antibody was generated against full length COX4I2 protein and cross reacts with COX4I1. This antibody is specific to COX4I2.