Tested Applications
| Positive WB detected in | HL-60 cells, A549 cells, Caco-2 cells, HeLa cells, HepG2 cells |
| Positive IHC detected in | human liver cancer tissue, human kidney tissue, human breast cancer tissue, human colon cancer tissue, human placenta tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11425-1-AP targets COX6B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1984 Product name: Recombinant human COX6B1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-86 aa of BC001015 Sequence: MAEDMETKIKNYKTAPFDSRFPNQNQTRNCWQNYLDFHRCQKAMTAKGGDISVCEWYQRVYQSLCPTSWVTDWDEQRAEGTFPGKI Predict reactive species |
| Full Name | cytochrome c oxidase subunit Vib polypeptide 1 (ubiquitous) |
| Calculated Molecular Weight | 80 aa, 10 kDa |
| Observed Molecular Weight | 10-13 kDa |
| GenBank Accession Number | BC001015 |
| Gene Symbol | COX6B1 |
| Gene ID (NCBI) | 1340 |
| RRID | AB_2085449 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14854 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
COX6B1 (Cytochrome c oxidase subunit 6B1) is also named as COX6B and belongs to the cytochrome c oxidase subunit 6B family. It connects the two COX monomers into the physiological dimeric form. Defects in COX6B1 are a cause of mitochondrial complex IV deficiency (MT-C4D).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for COX6B1 antibody 11425-1-AP | Download protocol |
| IHC protocol for COX6B1 antibody 11425-1-AP | Download protocol |
| WB protocol for COX6B1 antibody 11425-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The COX6B1 Polyclonal antibody (Cat# 11425-1-AP) has 16 total citations across 14 journals, including Cell Metabolism, Protein & Cell, Science Advances, EMBO Reports, Journal of Biological Chemistry, and Human Reproduction. Research fields span mitochondrial oxidative phosphorylation, ferroptosis, neurodegeneration (Parkinson's disease), cancer (gastric/glioma), reproductive biology, pulmonary hypertension, and mitochondriopathies.
| Species | Application | Title |
|---|---|---|
Cell Metab Two independent respiratory chains adapt OXPHOS performance to glycolytic switch | ||
Protein Cell Isolation and Proteomics of Human Skeletal Muscle Lipid Droplets Identify Anchored Mitochondria and Associated TPD52L2 | ||
Pharmacol Res Discovery of KMT5A repressed miR-99b cluster with potential to restore chemotherapy sensitivity in gastric cancer by regulating mitochondrial complex II and affecting OXPHOS. | ||
Environ Int Bisphenol S impairs mitochondrial function by targeting Myo19/oxidative phosphorylation pathway contributing to axonal and dendritic injury | ||
Hum Reprod Posttranslational lysine 2-hydroxyisobutyrylation of human sperm tail proteins affects motility. |
