CPLX1 Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB, IP |
| Cited applications | WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human |
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Recommended dilution | WB: 1:500-1:5000 |
IP = Immunoprecipitation
IP result of CPLX1 antibody (10246-2-AP for IP and Detection) with mouse liver tissue lysate.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10246-2-AP(CPLX1 Antibody) at dilution of 1:1500
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | CPLX1 fusion protein ag0387 | Full name | complexin 1 |
|
Calculated molecular weight |
15kd |
Observed molecular
weight |
15-20 kDa |
|
GenBank accession number |
BC002471 | Gene ID (NCBI) | 10815 |
| Gene symbol | CPLX1 | Synonyms | CPX-I; CPX1 |
Complexin 1 (CPLX1) is a member of the complexin/synaphin gene family, which are cytosolic proteins that function in synaptic vesicle exocytosis. Complexins are enriched in neurons where they colocalize with syntaxin and SNAP-25. Complexins bind weakly to syntaxin alone and not at all to synaptobrevin and SNAP-25, but strongly to the SNAP receptor-core complex composed of these three molecules. Compexins also compete with alpha-SNAP for binding to the core complex but not with other interacting molecules, including synaptotagmin I, and regulate the sequential interactions of alpha-SNAP and synaptotagmins with the SNAP receptor during exocytosis. CLPX1 binds to the SNAP receptor complex and disrupts it, leading to the release of transmitters. Alterations of complexins may contribute to the molecular substrate for abnormalities of neural connectivity in severe mental disorders. |
