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  • Featured Product
  • KD/KO Validated

CROP Polyclonal antibody

CROP Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14504-1-AP

Synonyms

CRE associated protein 1, CREAP 1, CREAP1, CROP, Luc7 like protein 3, LUC7A, LUC7L3, O48, OA48 18

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CROP Polyclonal antibody (14504-1-AP)

    WB analysis of Jurkat using 14504-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CROP Polyclonal antibody (14504-1-AP)

    WB analysis of HepG2 using 14504-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CROP Polyclonal antibody (14504-1-AP)

    WB analysis of mouse brain using 14504-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CROP Polyclonal antibody (14504-1-AP)

    WB analysis of HepG2 using 14504-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CROP Polyclonal antibody (14504-1-AP)

    WB analysis of Jurkat using 14504-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14504-1-AP (CROP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using CROP Polyclonal antibody (14504-1-AP)

    IP experiment of HepG2 using 14504-1-AP

    IP result of anti-CROP (IP:14504-1-AP, 4ug; Detection:14504-1-AP 1:500) with HepG2 cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CROP Polyclonal antibody (14504-1-AP)

    IHC staining of human pancreas cancer using 14504-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14504-1-AP (CROP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CROP Polyclonal antibody (14504-1-AP)

    IHC staining of human pancreas cancer using 14504-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14504-1-AP (CROP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CROP Polyclonal antibody (14504-1-AP)

    IHC staining of human pancreas cancer using 14504-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CROP Polyclonal antibody (14504-1-AP)

    IHC staining of human pancreas cancer using 14504-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using CROP Polyclonal antibody (14504-1-AP)

    IHC staining of human ovary using 14504-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using CROP Polyclonal antibody (14504-1-AP)

    IHC staining of human ovary using 14504-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 14504-1-AP (CROP Antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CROP Polyclonal antibody (14504-1-AP)

    IF Staining of HepG2 using 14504-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 14504-1-AP (CROP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, HepG2 cells, mouse brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman pancreas cancer tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14504-1-AP targets CROP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CROP fusion protein Ag5961
Full Name cisplatin resistance-associated overexpressed protein
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 55-58 kDa
GenBank Accession NumberBC056409
Gene Symbol CROP
Gene ID (NCBI) 51747
RRIDAB_2229967
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CROP, a putative SR protein, contain cystine/histidine motifs and leucine zipper-like repeats in the N-terminal half and consists mostly of charged and polar amino acids in the C-terminal half. CROP has a high expression in heart, brain, pancreas, thymus, and weak in lung, live and kidney. As the human homologue of yeast Luc7p, CROP is supported to be participated in 5'-splice site recognize and is essential for vegetative growth. This is a rabbit polyclonal antibody raised against part of N-terminus of CROP of human origin, and can recognize a ~58kDa band of CROP.

Protocols

Product Specific Protocols
WB protocol for CROP antibody 14504-1-APDownload protocol
IHC protocol for CROP antibody 14504-1-APDownload protocol
IF protocol for CROP antibody 14504-1-APDownload protocol
IP protocol for CROP antibody 14504-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Genet

Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.

Authors - Emily M Schleicher
  • KD Validated
View Article
humanWB

Cell Rep

Functional analyses of human LUC7-like proteins involved in splicing regulation and myeloid neoplasms.

Authors - Noah J Daniels
  • KD Validated
View Article