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WB analysis using 15724-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15724-1-AP
WB result of CRTAP antibody (15724-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRTAP transfected HeLa cells.
WB analysis of human colon using 15724-1-AP
human colon tissue were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human stomach using 15724-1-AP
human stomach tissue were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15724-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 15724-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 15724-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of ROS1728 using 15724-1-AP
ROS1728 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 15724-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 15724-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 15724-1-AP (CRTAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 15724-1-AP
IP result of anti-CRTAP (IP:15724-1-AP, 4ug; Detection:15724-1-AP 1:500) with HEK-293 cells lysate 3000 ug.
IHC staining of human kidney using 15724-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15724-1-AP (CRTAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 15724-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15724-1-AP (CRTAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 15724-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CRTAP antibody (15724-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin was stained using CL594-phalloidin (red) and DNA was stained by DAPI (blue).

Featured Product
KD/KO Validated

CRTAP Polyclonal antibody

CRTAP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15724-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Cartilage-associated protein, CASP, LEPREL3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HT-1080 cells, HEK-293 cells, HeLa cells, human colon tissue, human stomach tissue, NIH/3T3 cells, ROS1728 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below

Product Information

15724-1-AP targets CRTAP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8459 Product name: Recombinant human CRTAP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 50-401 aa of BC008745 Sequence: HALDKYSGEHWAESVGYLEISLRLHRLLRDSEAFCHRNCSAAPQPEPAAGLASYPELRLFGGLLRRAHCLKRCKQGLPAFRQSQPSRDVLADFQRREPYKFLQFAYFKANNLPKAIAAAHTFLLKHPDDEMMKRNMAYYKSLPGAEDYIKDLETKSYESLFIRAVRAYNGENWRTSITDMELALPDFFKAFYECLAACEGSREIKDFKDFYLSIADHYVEVLECKIQCEENLTPVIGGYPVEKFVATMYHYLQFAYYKLNDLKNAAPCAVSYLLFDQNDKVMQQNLVYYQYHRDTWGLSDEHFQPRPEAVQFFNVTTLQKELYDFAKENIMDDDEGEVVEYVDDLLELEETS Predict reactive species
Full Name	cartilage associated protein
Calculated Molecular Weight	46.5 kDa
Observed Molecular Weight	43-47 kDa
GenBank Accession Number	BC008745
Gene Symbol	CRTAP
Gene ID (NCBI)	10491
RRID	AB_2261089
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75718
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for CRTAP antibody 15724-1-AP	Download protocol
IHC protocol for CRTAP antibody 15724-1-AP	Download protocol
IP protocol for CRTAP antibody 15724-1-AP	Download protocol
WB protocol for CRTAP antibody 15724-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Med Oncol Data-independent acquisition (DIA) mass spectrometry reveals related proteins involved in the occurrence of early intestinal-type gastric cancer Authors - Liangshun Zhang View Article
human	WB	Nat Commun The structural basis for the collagen processing by human P3H1/CRTAP/PPIB ternary complex Authors - Wenguo Li View Article