CSAD Polyclonal antibody

CSAD Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 22870-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Synonyms

Aspartate 1-decarboxylase, CSD, Cysteine-sulfinate decarboxylase, EC:4.1.1.11, EC:4.1.1.29

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Proteintech Guarantee

Filter: AllIHCIF/ICC

IHC staining of human liver using 22870-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22870-1-AP (CSAD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 22870-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22870-1-AP (CSAD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 22870-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 22870-1-AP (CSAD antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 22870-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 22870-1-AP (CSAD antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas using 22870-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 22870-1-AP (CSAD antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human pancreas using 22870-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 22870-1-AP (CSAD antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of MCF-7 using 22870-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CSAD antibody (22870-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (2)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive IHC detected in	human liver tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22870-1-AP targets CSAD in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15828 Product name: Recombinant human CSAD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 344-493 aa of BC098278 Sequence: DVALDTGDKVVQCGRRVDCLKLWLMWKAQGDQGLERRIDQAFVLARYLVEEMKKREGFELVMEPEFVNVCFWFVPPSLRGKQESPDYHERLSKVAPVLKERMVKEGSMMIGYQPHGTRGNFFRVVVANSALTCADMDFLLNELERLGQDL Predict reactive species
Full Name	cysteine sulfinic acid decarboxylase
Calculated Molecular Weight	493 aa, 55 kDa
GenBank Accession Number	BC098278
Gene Symbol	CSAD
Gene ID (NCBI)	51380
RRID	AB_11183049
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y600
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for CSAD antibody 22870-1-AP	Download protocol
IHC protocol for CSAD antibody 22870-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
mouse	IHC	Hepatol Res Oncogenic Signaling Activation and Potential Biomarkers in Congestive Hepatopathy Revealed by Proteomic Analysis. Authors - Yoshihito Morimoto View Article
		Cell Mol Immunol Intestinal taurine acts as a novel immunometabolic modulator of IBD by degrading redundant mitochondrial RNA Authors - Le-Xi Wu View Article