Tested Applications
| Positive IP detected in | THP-1 cells |
| Positive IHC detected in | mouse brain tissue, human brain tissue, human cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
| Positive IF/ICC detected in | THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25949-1-AP targets CSF1R/CD115 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22845 Product name: Recombinant human CSF1R protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 20-369 aa of BC047521 Sequence: IPVIEPSVPELVVKPGATVTLRCVGNGSVEWDGPPSPHWTLYSDGSSSILSTNNATFQNTGTYRCTEPGDPLGGSAAIHLYVKDPARPWNVLAQEVVVFEDQDALLPCLLTDPVLEAGVSLVRVRGRPLMRHTNYSFSPWHGFTIHRAKFIQSQDYQCSALMGGRKVMSISIRLKVQKVIPGPPALTLVPAELVRIRGEAAQIVCSASSVDVNFDVFLQHNNTKLAIHQQSDFHNNRYQKVLTLNLDQVDFQHAGNYSCVASNVQGKHSTSMFFRVVESAYLNLSSEQNLIQEVTVGEGLNLKVMVEAYPGLQGFNWTYLGPFSDHQPEPKLANVTTKDTYRHTFTLSLP Predict reactive species |
| Full Name | colony stimulating factor 1 receptor |
| Calculated Molecular Weight | 972 aa, 108 kDa |
| GenBank Accession Number | BC047521 |
| Gene Symbol | CSF1R/CD115 |
| Gene ID (NCBI) | 1436 |
| RRID | AB_2880306 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07333 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CSF1R (colony-stimulating factor 1 receptor) belongs to the type III protein tyrosine kinase receptor family. CSF1R-mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular (PMID: 28716061).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CSF1R/CD115 antibody 25949-1-AP | Download protocol |
| IHC protocol for CSF1R/CD115 antibody 25949-1-AP | Download protocol |
| IP protocol for CSF1R/CD115 antibody 25949-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Arthritis Res Ther Integrating transcriptome-wide study and mRNA expression profiles yields novel insights into the biological mechanism of chondropathies. | ||
CNS Neurosci Ther A novel chemokine-based signature for prediction of prognosis and therapeutic response in glioma | ||
J Nat Med Insight of action mechanism of Astragaloside IV for relieving of cerebral ischemic injury in a rat model of middle cerebral artery occlusion reperfusion via proteomics and network pharmacology | ||
FASEB J Cellular Landscape of Synovial Chondromatosis Synovium Revealed by Single-Cell RNA Sequencing | ||
Cell Rep Med Tumor heterogeneity and tumor-microglia interactions in primary and recurrent IDH1-mutant gliomas |
Reviews
What customers say
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jaouhara (Verified Customer) (12-05-2023) | Used in synovium PFA-fixed slides to detect macrophages. Works well.
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