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  • Featured Product
  • KD/KO Validated

CST1 Polyclonal antibody

CST1 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16025-1-AP

Synonyms

CST1, Cystain SA I, Cystatin 1, cystatin SN, Salivary cystatin SA 1

Validation Data Gallery

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  • Western Blot (WB) analysis of human saliva using CST1 Polyclonal antibody (16025-1-AP)

    WB analysis of human saliva using 16025-1-AP

    human saliva were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using CST1 Polyclonal antibody (16025-1-AP)

    WB analysis of COLO 320 using 16025-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human adrenal gland tissue using CST1 Polyclonal antibody (16025-1-AP)

    WB analysis of human adrenal gland using 16025-1-AP

    human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using CST1 Polyclonal antibody (16025-1-AP)

    WB analysis of human testis using 16025-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of MCF-7 cells using CST1 Polyclonal antibody (16025-1-AP)

    FC experiment of MCF-7 using 16025-1-AP

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human CST1 (16025-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inhuman saliva tissue, human testis tissue, COLO 320 cells, human adrenal gland tissue
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16025-1-AP targets CST1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CST1 fusion protein Ag8862
Full Name cystatin SN
Calculated Molecular Weight 141 aa, 16 kDa
Observed Molecular Weight 14-16 kDa
GenBank Accession NumberBC021225
Gene Symbol CST1
Gene ID (NCBI) 1469
RRIDAB_10916385
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CST1 antibody 16025-1-APDownload protocol
FC protocol for CST1 antibody 16025-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cell Rep Med

Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application

Authors - Xiaoxue Ren
View Article
humanIHC,IF

Nat Commun

Integrated single-cell transcriptome analysis reveals heterogeneity of esophageal squamous cell carcinoma microenvironment.

Authors - Huy Q Dinh
View Article
humanWB

Cell Res

Differential secretome analysis reveals CST6 as a suppressor of breast cancer bone metastasis.

Authors - Jin Lei L
View Article
humanWB,IHC,IF,IP

Oncogene

CST1 inhibits ferroptosis and promotes gastric cancer metastasis by regulating GPX4 protein stability via OTUB1

Authors - Dongbao Li
  • KD Validated
View Article
humanWB

Mol Oncol

Cancer-associated fibroblasts educate normal fibroblasts to facilitate cancer cell spreading and T cell suppression.

Authors - Go Itoh
View Article
humanIHC

Aging (Albany NY)

Transcriptional dysregulation of TRIM29 promotes colorectal cancer carcinogenesis via pyruvate kinase-mediated glucose metabolism.

Authors - Jing Han
View Article