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CTAGE6 Polyclonal antibody

CTAGE6 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 17438-1-AP

Synonyms

cTAGE family member 6, CTAGE family, member 6, CTAGE6, CTAGE6P, Putative protein cTAGE 6

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CTAGE6 Polyclonal antibody (17438-1-AP)

    WB analysis of HeLa using 17438-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17438-1-AP (CTAGE6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using CTAGE6 Polyclonal antibody (17438-1-AP)

    WB analysis of MCF-7 using 17438-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 17438-1-AP (CTAGE6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using CTAGE6 Polyclonal antibody (17438-1-AP)

    IHC staining of human testis using 17438-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17438-1-AP (CTAGE6 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using CTAGE6 Polyclonal antibody (17438-1-AP)

    IHC staining of human testis using 17438-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17438-1-AP (CTAGE6 antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17438-1-AP is CTAGE6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CTAGE6 fusion protein Ag11511
Full Name CTAGE family, member 6
Calculated Molecular Weight 732 aa, 83 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC043153
Gene Symbol CTAGE6
Gene ID (NCBI) 340307
RRIDAB_2878407
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CTAGE6 antibody 17438-1-APDownload protocol
IHC protocol for CTAGE6 antibody 17438-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols