CUL4B Antibody
Rabbit Polyclonal
Catalog number: 12916-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF, IP |
| Cited applications | IF, IHC, IP, WB |
| Species specificity | human, mouse; other species not tested. |
| Cited species | mouse, rat |
| Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells, mouse testis tissue, mouse thymus tissue, SH-SY5Y cells, human testis tissue |
| Positive IHC detected in | human gliomas |
| Positive IF detected in | HepG2 cells |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:50-1:200 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
IP resul of anti-CUL4B (12916-1-AP for IP and Detection) with HeLa cell lysate.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12916-1-AP(CUL4B Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human gliomas using 12916-1-AP(CUL4B Antibody) at Dilution 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using CUL4B antibody 12916-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | CUL4B fusion protein ag3563 | Full name | cullin 4B |
|
Calculated molecular weight |
913aa,104kd |
Observed molecular
weight |
102 kDa |
|
GenBank accession number |
BC036216 | Gene ID (NCBI) | 8450 |
| Gene symbol | CUL4B | Synonyms | DKFZp686F1470; KIAA0695; MRXHF2; MRXSC; SFM2 |
Background
Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329). |
