CUL4B Antibody
Rabbit Polyclonal
Catalog number: 20882-1-AP
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Applications
| Tested applications | ELISA, WB, IF |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | mouse brain tissue, mouse thymus tissue, SH-SY5Y cells, Y79 cells, human testis tissue |
| Positive IF detected in | Hela cells |
| Recommended dilution | WB: 1:1000-1:10000 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
mouse brain tissue were subjected to SDS PAGE followed by western blot with 20882-1-AP(CUL4B Antibody) at dilution of 1:2000
Immunofluorescent analysis of Hela cells, using CUL4B antibody 20882-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | CUL4B fusion protein ag15024 | Full name | cullin 4B |
|
Calculated molecular weight |
913aa,104kd |
Observed molecular
weight |
102kd |
|
GenBank accession number |
BC036216 | Gene ID (NCBI) | 8450 |
| Gene symbol | CUL4B | Synonyms | DKFZp686F1470; KIAA0695; MRXHF2; MRXSC; SFM2 |
Background
ces are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2. This antibody can bind CUL4B only. |
