CUL4B Antibody
Mouse Monoclonal
Catalog number: 60151-1-Ig
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Applications
| Tested applications | ELISA, WB |
| Cited applications | |
| Species specificity | human; other species not tested. |
| Cited species | |
| Positive WB detected in | 293 cells |
| Recommended dilution | WB: 1:500-1:5000 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
293 cells were subjected to SDS PAGE followed by western blot with 60151-1-Ig(CUL4B Antibody) at dilution of 1:1000
Product Information
| Source | Mouse | Purification method | Caprylic acid/ammonium sulfate precipitation |
| Isotype | IgG1 | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | CUL4B fusion protein ag3563 | Full name | cullin 4B |
|
Calculated molecular weight |
913aa,104kd |
Observed molecular
weight |
102KD |
|
GenBank accession number |
BC036216 | Gene ID (NCBI) | 8450 |
| Gene symbol | CUL4B | Synonyms | DKFZp686F1470; KIAA0695; MRXHF2; MRXSC; SFM2 |
Background
Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2. |
