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CWF19L2 Polyclonal antibody

CWF19L2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24872-1-AP

Synonyms

CWF19 like protein 2, CWF19L2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CWF19L2 Polyclonal antibody (24872-1-AP)

    WB analysis of HeLa using 24872-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24872-1-AP (CWF19L2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CWF19L2 Polyclonal antibody (24872-1-AP)

    WB analysis of HEK-293 using 24872-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24872-1-AP (CWF19L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CWF19L2 Polyclonal antibody (24872-1-AP)

    WB analysis of Jurkat using 24872-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24872-1-AP (CWF19L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using CWF19L2 Polyclonal antibody (24872-1-AP)

    WB analysis of PC-3 using 24872-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 24872-1-AP (CWF19L2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CWF19L2 Polyclonal antibody (24872-1-AP)

    IHC staining of human kidney using 24872-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24872-1-AP (CWF19L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CWF19L2 Polyclonal antibody (24872-1-AP)

    IHC staining of human kidney using 24872-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24872-1-AP (CWF19L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, PC-3 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24872-1-AP is CWF19L2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CWF19L2 fusion protein Ag21738
Full Name CWF19-like 2, cell cycle control (S. pombe)
Calculated Molecular Weight 894 aa, 104 kDa
Observed Molecular Weight 103 kDa
GenBank Accession NumberBC110442
Gene Symbol CWF19L2
Gene ID (NCBI) 143884
RRIDAB_2879769
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CWF19L2 antibody 24872-1-APDownload protocol
IHC protocol for CWF19L2 antibody 24872-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols