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WB analysis of mouse lung using 20837-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 20837-1-AP (CXorf22 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 20837-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20837-1-AP (CXorf22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 20837-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20837-1-AP (CXorf22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 20837-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 20837-1-AP (CXorf22 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung using 20837-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 20837-1-AP (CXorf22 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human spleen using 20837-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 20837-1-AP (CXorf22 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human spleen using 20837-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 20837-1-AP (CXorf22 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of mouse kidney using 20837-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using CFAP47 antibody (20837-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CFAP47 Polyclonal antibody

Name: CFAP47 Polyclonal antibody
Brand: Proteintech
SKU: 20837-1-AP

CFAP47 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 20837-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, CoIP, ELISA

Synonyms

CXorf22, CHDC2, Cilia- and flagella-associated protein 47, CXorf30, CXorf59

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse lung tissue
Positive IHC detected in	human lung cancer tissue, human lung tissue, human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse kidney tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IF	See 2 publications below
CoIP	See 1 publications below

Product Information

20837-1-AP targets CFAP47 in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag14910 Product name: Recombinant human CXorf22 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC027936 Sequence: MNTQKGSLTINVHRGSLAMSIQRGSLVPRDMDSSGRDMQLRVIPAEVKFLDTMAGRVYRLPITVHNICRWNQKIRFKEPVKPQFKLMLTSLDKELASGLQMTAMVEYHPDKDEDTFDRLLISIENKTTEIPLIGLIPSCQLEIESVVNFGTLVANSKVYSKEITITNHGKAPGIFKAEYHGQLPILIFPTSGIVDAKSSMVIKVDFCADQPRIVDEEAIVILQGQPEMLLSIKAHVVEQIIELLSMSSDRRLECIHFGPVFFGSSKIKHARVYNNSPEPINWVAIIQDDAVGEELGTDIQQRTDIALNNLTYIRKIKNIDTTIIISCLPNEGTLQPYQKTVITFCFTPKLM Predict reactive species
Full Name	chromosome X open reading frame 22
Calculated Molecular Weight	976 aa, 110 kDa
Observed Molecular Weight	100-110 kDa
GenBank Accession Number	BC027936
Gene Symbol	CFAP47/CXorf22
Gene ID (NCBI)	170063
RRID	AB_11232036
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6ZTR5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CFAP47 (Cilia- and flagella-associated protein 47), formerly known as CXorf22, CXorf59, is located on the human chromosome X and highly expressed in the testis and encodes a cilia- and flagella-associated protein. CFAP47 was expressed in primary cilia of human kidney tubules, and knockout (KO) mice exhibited vacuolation of tubular cells and tubular dilation, providing evidence that CFAP47 is a causative gene involved in cyst formation (PMID: 33472045, 38633811). CFAP47-mutated male mice are sterile, with decreased sperm motility and abnormal flagella morphology (PMID: 37424856).

Protocols

Product Specific Protocols
IF protocol for CFAP47 antibody 20837-1-AP	Download protocol
IHC protocol for CFAP47 antibody 20837-1-AP	Download protocol
WB protocol for CFAP47 antibody 20837-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse,human	WB,IF,CoIP	Mol Hum Reprod WDR87 interacts with CFAP47 protein in the middle piece of spermatozoa flagella to participate in sperm tail assembly Authors - Hong-Qing Liao View Article
human	IF	medRxiv CFAP47 is a novel causative gene implicated in X-linked polycystic kidney disease Authors - Takayasu Mori View Article