Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, mouse kidney tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IP | See 1 publications below |
Product Information
24021-1-AP targets CXorf56 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19856 Product name: Recombinant human CXorf56 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-222 aa of BC023506 Sequence: MPKVVSRSVVCSDTRDREEYDDGEKPLHVYYCLCGQMVLVLDCQLEKLPMRPRDRSRVIDAAKHAHKFCNTEDEETMYLRRPEGIERQYRKKCAKCGLPLFYQSQPKNAPVTFIVDGAVVKFGQGFGKTNIYTQKQEPPKKVMMTKRTKDMGKFSSVTVSTIDEEEEEIEAREVADSYAQNAKVIEKQLERKGMSKRRLQELAELEAKKAKMKGTLIDNQFK Predict reactive species |
| Full Name | chromosome X open reading frame 56 |
| Calculated Molecular Weight | 222 aa, 26 kDa |
| Observed Molecular Weight | 26-30 kDa |
| GenBank Accession Number | BC023506 |
| Gene Symbol | CXorf56 |
| Gene ID (NCBI) | 63932 |
| RRID | AB_2879404 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q9H5V9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CxORF56 (also known as STEEP) is an evolutionarily highly conserved protein whose function is unknown. Recently CxORF56 has been identified as a protein that interacts with STING, and it is essential for STING ER exit. CxORF56-dependent STING ER exit is essential for STING signaling in antiviral defense and in inflammatory diseases.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CXorf56 antibody 24021-1-AP | Download protocol |
| IP protocol for CXorf56 antibody 24021-1-AP | Download protocol |
| WB protocol for CXorf56 antibody 24021-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EBioMedicine A STING antagonist modulating the interaction with STIM1 blocks ER-to-Golgi trafficking and inhibits lupus pathology. | ||
J Virol The Papain-Like Protease of Porcine Reproductive and Respiratory Syndrome Virus Impedes STING Translocation from the Endoplasmic Reticulum to the Golgi Apparatus by Deubiquitinating STIM1 | ||
NPJ Breast Cancer Inhibition of CXorf56 promotes PARP inhibitor-induced cytotoxicity in triple-negative breast cancer | ||
Nat Commun Cholesterol-binding motifs in STING that control endoplasmic reticulum retention mediate anti-tumoral activity of cholesterol-lowering compounds |
