CYB5R3 AntibodyRabbit Polyclonal
|Tested applications||ELISA, WB, IHC, IF, IP|
|Cited applications||IF, IHC, WB|
|Species specificity||human, mouse, rat; other species not tested.|
|Cited species||hamster, rat|
|Positive WB detected in||HepG2 cells, A431 cells, HeLa cells, human liver tissue, human lung tissue, human placenta tissue, MCF7 cells, mouse placenta tissue|
|Positive IHC detected in||human lung cancer|
|Positive IF detected in||Hela cells|
|Recommended dilution||WB: 1:500-1:5000|
IP = Immunoprecipitation
- IP result of CYB5R3 antibody (10894-1-AP for IP and Detection) with HepG2 cell lysate.
- HepG2 cells were subjected to SDS PAGE followed by western blot with 10894-1-AP(CYB5R3 Antibody) at dilution of 1:1000
- Immunohistochemistry of paraffin-embedded human lung cancer using 10894-1-AP(CYB5R3 Antibody) at Dilution 1:50 (under 10x lens)
- Immunofluorescent analysis of HepG2 cells, using CYB5R3 antibody 10894-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
|Source||Rabbit||Purification method||Antigen affinity purification|
|Isotype||IgG||Storage||PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.|
|Immunogen||CYB5R3 fusion protein ag1339||Full name||cytochrome b5 reductase 3|
|BC004821||Gene ID (NCBI)||1727|
|Gene symbol||CYB5R3||Synonyms||B5R; DIA1|
CYB5R3, also named as DIA1 and B5R, belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. It plays an essential role in the mitochondrial metabolism by its contribution to cellular redox homeostasis. Overstimulation of CYB5R3 associated with lipid rafts can account for the overshot of plasma membrane-focalized superoxide anion production that triggers the entry of CGN in the irreversible phase of apoptosis.This antibody is specific to CYB5R3.