Tested Applications
| Positive WB detected in | HeLa cells, NCCIT cells, HepG2 cells, MCF-7 cells |
| Positive IHC detected in | human rectal cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26457-1-AP targets Ch-TOG in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24875 Product name: Recombinant human CKAP5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-349 aa of BC120869 Sequence: MGDDSEWLKLPVDQKCEHKLWKARLSGYEEALKIFQKIKDEKSPEWSKFLGLIKKFVTDSNAVVQLKGLEAALVYVENAHVAGKTTGEVVSGVVSKVFNQPKAKAKELGIEICLMYIEIEKGEAVQEELLKGLDNKNPKIIVACIETLRKALSEFGSKIILLKPIIKVLPKLFESREKAVRDEAKLIAVEIYRWIRDALRPPLQNINSVQLKELEEEWVKLPTSAPRPTRFLRSQQELEAKLEQQQSAGGDAEGGGDDGDEVPQIDAYELLEAVEILSKLPKDFYDKIEAKKWQERKEALESVEVLIKNPKLEAGDYADLVKALKKVVGKDTNVMLVALAAKCLTGLAV Predict reactive species |
| Full Name | cytoskeleton associated protein 5 |
| Observed Molecular Weight | 220 kDa |
| GenBank Accession Number | BC120869 |
| Gene Symbol | Ch-TOG |
| Gene ID (NCBI) | 9793 |
| RRID | AB_2880520 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14008 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ch-TOG (colonic hepatic tumor-overexpressed gene), also known as XMAP215 or CKAP5, is a microtubule polymerase which can promotes cytoplasmic microtubule nucleation and elongation. Through interacting with Aurora-A and TACC3, it plays a major role in organizing spindle poles.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Ch-TOG antibody 26457-1-AP | Download protocol |
| IHC protocol for Ch-TOG antibody 26457-1-AP | Download protocol |
| WB protocol for Ch-TOG antibody 26457-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Ch-TOG Polyclonal antibody (Cat# 26457-1-AP) has 2 total citations. These citations appear in Clinical & Translational Medicine (2025) and Cell Reports (2022). The research fields covered include microtubule dynamics and acetylation in oocyte spindle defects, as well as mitotic spindle assembly and Cdk1 regulation. Both studies utilized this antibody for Western blot and/or immunofluorescence applications in human samples.
| Species | Application | Title |
|---|---|---|
Clin Transl Med Pathogenic variants of TUBB8 cause oocyte spindle defects by disrupting with EB1/CAKP5 interactions and potential treatment targeting microtubule acetylation through HDAC6 inhibition | ||
