COL4A2-specific Polyclonal antibody

COL4A2-specific Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55131-1-AP

Synonyms

COL4A2, Collagen alpha 2(IV) chain, Collagen Type IV, collagen, type IV, alpha 2



Tested Applications

Positive WB detected inHeLa cells, mouse lung tissue, rat lung tissue
Positive IHC detected inhuman kidney tissue, human breast hyperplasia tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55131-1-AP targets COL4A2-specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name collagen, type IV, alpha 2
Calculated Molecular Weight 168 kDa
Observed Molecular Weight200 kDa
GenBank Accession NumberNM_001846
Gene Symbol COL4A2
Gene ID (NCBI) 1284
RRIDAB_2881273
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for COL4A2-specific antibody 55131-1-APDownload protocol
IHC protocol for COL4A2-specific antibody 55131-1-APDownload protocol
IF protocol for COL4A2-specific antibody 55131-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

EMBO J

Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress.

Authors - Simon Schwörer
human

EBioMedicine

Aspirin targets P4HA2 through inhibiting NF-κB and LMCD1-AS1/let-7g to inhibit tumour growth and collagen deposition in hepatocellular carcinoma.

Authors - Tianjiao Wang
IF

ACS Appl Mater Interfaces

Novel Thermosensitive Hydrogel Promotes Spinal Cord Repair by Regulating Mitochondrial Function.

Authors - Yi Li
humanIF

Biomaterials

Tumor-specific nitric oxide generator to amplify peroxynitrite based on highly penetrable nanoparticles for metastasis inhibition and enhanced cancer therapy.

Authors - Menghao Shi
humanIHC

Biofabrication

Engineering microvasculature by 3D bioprinting of prevascularized spheroids in photo-crosslinkable gelatin.

Authors - Lise De Moor
IHC

Oxid Med Cell Longev

Pretreatment with Roxadustat (FG-4592) Attenuates Folic Acid-Induced Kidney Injury through Antiferroptosis via Akt/GSK-3β/Nrf2 Pathway.

Authors - Xue Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Pankhuri (Verified Customer) (08-22-2022)

Nice clean antibody. Did IF for collagen in NSCs organoid. The collagen fibres in the ECM of organoid were stained beautifully.

  • Applications: Immunofluorescence
  • Cell Tissue Type: NSC organoid
COL4A2-specific Antibody Immunofluorescence validation ( dilution) in NSC organoid (Cat no:55131-1-AP)
FH

Lindsey (Verified Customer) (05-24-2021)

BULLETPROOF ANTIBODY!

  • Applications: Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human kidney (FFPE)
COL4A2-specific Antibody Immunohistochemistry,Immunofluorescence validation (1:500 dilution) in Human kidney (FFPE) (Cat no:55131-1-AP)
FH

Malak (Verified Customer) (02-24-2021)

This antibody works very well

  • Applications: Western Blot
FH

Joshua (Verified Customer) (04-28-2019)

Vascular endothelial cells in 3D culture were fixed in paraformaldehdye and stained for collagen IV (red). DRAQ7 used as a counterstain (blue). As expected, collagen IV localized to the vascular basement membranes.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HMEC1
COL4A2-specific Antibody Immunofluorescence, validation (1:500 dilution) in HMEC1 (Cat no:55131-1-AP)