Tested Applications
| Positive WB detected in | mouse brain tissue, HepG2 cells, human heart tissue, mouse liver tissue, mouse heart tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 8 publications below |
| WB | See 21 publications below |
| IHC | See 11 publications below |
| IF | See 10 publications below |
| CoIP | See 1 publications below |
Product Information
14876-1-AP targets DAAM1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, canine, goat, xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6648 Product name: Recombinant human DAAM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 715-1068 aa of BC064999 Sequence: KDMLEQLLKFVPEKSDIDLLEEHKHELDRMAKADRFLFEMSRINHYQQRLQSLYFKKKFAERVAEVKPKVEAIRSGSEEVFRSGALKQLLEVVLAFGNYMNKGQRGNAYGFKISSLNKIADTKSSIDKNITLLHYLITIVENKYPSVLNLNEELRDIPQAAKVNMTELDKEISTLRSGLKAVETELEYQKSQPPQPGDKFVSVVSQFITVASFSFSDVEDLLAEAKDLFTKAVKHFGEEAGKIQPDEFFGIFDQFLQAVSEAKQENENMRKKKEEEERRARMEAQLKEQRERERKMRKAKENSEESGEFDDLVSALRSGEVFDKDLSKLKRNRKRITNQMTDSSRERPITKLNF Predict reactive species |
| Full Name | dishevelled associated activator of morphogenesis 1 |
| Calculated Molecular Weight | 123 kDa |
| Observed Molecular Weight | 123 kDa |
| GenBank Accession Number | BC064999 |
| Gene Symbol | DAAM1 |
| Gene ID (NCBI) | 23002 |
| RRID | AB_2089444 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y4D1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Dishevelled-associated activator of morphogenesis 1 (Daam1) is a member of the formin protein family that is implicated in actin assembly. Daam1 also plays a critical role in mediating Wnt/Fz signaling pathway and regulates cell polarity during development and tissue homoeostasis. This antibody well recognizes the endogenous DAAM1 protein around 120 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DAAM1 antibody 14876-1-AP | Download protocol |
| IHC protocol for DAAM1 antibody 14876-1-AP | Download protocol |
| IP protocol for DAAM1 antibody 14876-1-AP | Download protocol |
| WB protocol for DAAM1 antibody 14876-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther C/EBPβ activation in vascular smooth muscle cells promotes hyperlipidemia-induced phenotypic transition and arterial stiffness | ||
J Cell Biol DAAM1 stabilizes epithelial junctions by restraining WAVE complex-dependent lateral membrane motility.
| ||
Ecotoxicol Environ Saf Prenatal Perfluorooctanoic Acid (PFOA) exposure causes reproductive toxicity by disrupting the formation of transzonal projections (TZPs) and down-regulating Wnt4/β-catenin signaling pathway in progeny | ||
Development Dishevelled-associated activator of morphogenesis 1 (Daam1) is required for heart morphogenesis.
| ||
Cell Prolif Formin homology domains of Daam1 bind to Fascin and collaboratively promote pseudopodia formation and cell migration in breast cancer. | ||
Cell Signal miR-613 inhibits cell migration and invasion by downregulating Daam1 in triple-negative breast cancer. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Vanina (Verified Customer) (11-11-2020) | Cell staining using 1:100 antibody dilution
 |
FH Vanina (Verified Customer) (11-11-2020) | Western blot using 20 µg of protein per lane.
 |
FH Tongbin (Verified Customer) (08-25-2020) | Although I didn't see any differences in Daam1 protein level between our wild type and knockout animals. This antibody was very specific and had a sharp ~125kD band.
 |
