Tested Applications
| Positive IHC detected in | human stomach cancer tissue, rat colon tissue, rat heart tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, U-251 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19540-1-AP targets ZIP kinase in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | death-associated protein kinase 3 |
| Calculated Molecular Weight | 53 kDa |
| Observed Molecular Weight | 37, 52 kDa |
| GenBank Accession Number | NM_001348 |
| Gene Symbol | DAPK3 |
| Gene ID (NCBI) | 1613 |
| RRID | AB_10860106 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43293 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Zipper-interacting protein (ZIP) kinase, also known as death-associated protein kinase 3 (DAPK3), is a
serine/threonine kinase that positively regulates mitochondrial-dependent apoptosis as well as autophagy
and acts as a tumor suppressor.
What is the molecular weight of ZIP kinase? Is ZIP kinase post-translationally modified?
The molecular weight of ZIP kinase is 52 kDa. ZIP kinase contains seven phosphorylation sites (six threonine
and one serine residues) that control its activity and localization (PMID: 24193917).
What is the subcellular localization of ZIP kinase?
ZIP kinase contains nuclear localization signal in its C-terminal part. ZIP kinase is present both in the cytoplasm
and in the nucleus. In the cytoplasm ZIP kinase interacts with actin cytoskeleton, regulating contraction of
smooth muscles (PMID: 16005610) and membrane blebbing (PMID: 11781833). In the nucleus it interacts
with several transcription factors regulating gene expression.
What is the tissue expression pattern of ZIP kinase?
ZIP kinase is ubiquitously expressed.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ZIP kinase antibody 19540-1-AP | Download protocol |
| IHC protocol for ZIP kinase antibody 19540-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
