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DAZ4 Polyclonal antibody

DAZ4 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 14065-1-AP

Synonyms

DAZ, DAZ1, DAZ4, deleted in azoospermia 4, pDP1680, pDP1681

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using DAZ4 Polyclonal antibody (14065-1-AP)

    WB analysis of mouse testis using 14065-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14065-1-AP (DAZ4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using DAZ4 Polyclonal antibody (14065-1-AP)

    WB analysis of mouse testis using 14065-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14065-1-AP (DAZ4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using DAZ4 Polyclonal antibody (14065-1-AP)

    WB analysis of human testis using 14065-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 14065-1-AP (DAZ4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using DAZ4 Polyclonal antibody (14065-1-AP)

    WB analysis of rat testis using 14065-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 14065-1-AP (DAZ4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse testis tissue, human testis tissue, rat testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14065-1-AP targets DAZ4 in WB,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DAZ4 fusion protein Ag5116
Full Name deleted in azoospermia 4
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 60-65 kDa
GenBank Accession NumberBC047617
Gene Symbol DAZ4
Gene ID (NCBI) 57135
RRIDAB_2878008
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DAZ4, encoded by the DAZ gene, is an RNA-binding protein with a role in spermatogenesis. DAZ4 is a testis-specific protein that expression restricted to premeiotic germ cells, particularly in spermatogonia. Defects in DAZ4 may be a cause of spermatogenic failure Y-linked type 2 (SPGFY2). It is a disorder resulting in the absence (azoospermia) or reduction (oligozoospermia) of sperm in the semen, leading to male infertility.

Protocols

Product Specific Protocols
WB protocol for DAZ4 antibody 14065-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols