• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

DBF4 Polyclonal antibody

DBF4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13076-1-AP

Synonyms

Activator of S phase kinase, ASK, CHIF, Chiffon homolog A, DBF4, DBF4 homolog (S. cerevisiae), DBF4A, Protein DBF4 homolog A, ZDBF1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of NIH/3T3 cells using DBF4 Polyclonal antibody (13076-1-AP)

    WB analysis of NIH/3T3 using 13076-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13076-1-AP (DBF4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using DBF4 Polyclonal antibody (13076-1-AP)

    WB analysis of NIH/3T3 using 13076-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13076-1-AP (DBF4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inNIH/3T3 cells, Jurkat cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13076-1-AP targets DBF4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DBF4 fusion protein Ag3771
Full Name DBF4 homolog (S. cerevisiae)
Calculated Molecular Weight77 kDa
Observed Molecular Weight 70-80 kDa
GenBank Accession NumberBC036045
Gene Symbol DBF4
Gene ID (NCBI) 10926
RRIDAB_10638788
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DBF4 antibody 13076-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols