Tested Applications
| Positive WB detected in | MCF-7 cells, A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26356-1-AP targets DCUN1D3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23730 Product name: Recombinant human DCUN1D3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-304 aa of BC040442 Sequence: MGQCVTKCKNPSSTLGSKNGDREPSNKSHSRRGAGHREEQVPPCGKPGGDILVNGTKKAEAATEACQLPTSSGDAGRESKSNAEESSLQRLEELFRRYKDEREDAILEEGMERFCNDLCVDPTEFRVLLLAWKFQAATMCKFTRKEFFDGCKAISADSIDGICARFPSLLTEAKQEDKFKDLYRFTFQFGLDSEEGQRSLHREIAIALWKLVFTQNNPPVLDQWLNFLTENPSGIKGISRDTWNMFLNFTQVIGPDLSNYSEDEAWPSLFDTFVEWEMERRKREGEGRGALSSGPEGLCPEEQT Predict reactive species |
| Full Name | DCN1, defective in cullin neddylation 1, domain containing 3 (S. cerevisiae) |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC040442 |
| Gene Symbol | DCUN1D3 |
| Gene ID (NCBI) | 123879 |
| RRID | AB_2880489 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IWE4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DCUN1D3, also named as SCCRO3, is proposed to function as a tumor suppressor. DCUN1D3 antagonizes DCUN1D1-mediated CUL1 neddylation by sequestering CUL1 at the cell membrane (PubMed:25349211). When overexpressed in transformed cells, DCUN1D3 may promote mesenchymal to epithelial-like changes and inhibit colony formation in soft agar (PubMed:25349211).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DCUN1D3 antibody 26356-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
