DDAH2 Antibody
Rabbit Polyclonal
Catalog number: 14966-1-AP
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Applications
| Tested applications | ELISA, WB, IHC |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | human heart tissue |
| Positive IHC detected in | human colon cancer |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:20-1:200 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
human heart tissue were subjected to SDS PAGE followed by western blot with 14966-1-AP(DDAH2 Antibody) at dilution of 1:800
Immunohistochemistry of paraffin-embedded human colon cancer using 14966-1-AP(DDAH2 Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human colon cancer using 14966-1-AP(DDAH2 Antibody) at Dilution 1:50 (under 40x lens)
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | DDAH2 fusion protein ag6831 | Full name | dimethylarginine dimethylaminohydrolase 2 |
|
Calculated molecular weight |
29kd |
Observed molecular
weight |
40kd |
|
GenBank accession number |
BC001435 | Gene ID (NCBI) | 23564 |
| Gene symbol | DDAH2 | Synonyms | DDAH; DDAHII; G6a; NG30 |
Background
The DDAH family of proteins is formed by two isoforms, DDAH-1 and DDAH-2, both of which utilize ADMA as their substrate and increase NOS activity. DDAH-2,also named as DDAH, G6A, NG30, is the most prevalent isoform in blood vessels and endothelium and is important for embryonic development. It has also been linked to other diseases, such as hypertension, cerebral hemorrhage, cardiac disease, and diabetes. In cultured human endothelial cells,DDAH-2 had been found only in the cytosol, but in the nuclei of rat vascular smooth muscle cells.(PMID:21898353). This antibody is specific to DDAH2. |
