DDB1 Antibody
Mouse Monoclonal
Catalog number: 66010-1-Ig
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Applications
| Tested applications | ELISA, WB, IHC, IF |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | Hela cells |
| Positive IHC detected in | human cervical cancer |
| Positive IF detected in | Hela cells |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
Hela cells were subjected to SDS PAGE followed by western blot with 66010-1-Ig(DDB1 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human cervical cancer using 66010-1-Ig(DDB1 Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human cervical cancer using 66010-1-Ig(DDB1 Antibody) at Dilution 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using DDB1 antibody 66010-1-lg at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
Product Information
| Source | Mouse | Purification method | Protein A purification |
| Isotype | IgG2b | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | DDB1 fusion protein ag17903 | Full name | damage-specific DNA binding protein 1, 127kDa |
|
Calculated molecular weight |
1140aa,127kd |
Observed molecular
weight |
127 kDa |
|
GenBank accession number |
BC011686 | Gene ID (NCBI) | 1642 |
| Gene symbol | DDB1 | Synonyms | DDBA; UV-DDB1; XAP1; XPCE; XPE; XPE-BF |
Background
DDB1, also named as XAP1, XPCe, DDBa and XPE-BF, belongs to the DDB1 family. It is required for DNA repair. DDB1 binds to DDB2 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The functional specificity of the DCX E3 ubiquitin-protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. This antibody is specific to DDB1. |
