Tested Applications
| Positive WB detected in | HepG2 cells, PC-3 cells, mouse liver tissue, mouse lung tissue, rat liver tissue, rat lung tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21445-1-AP targets DDRGK1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, bovine, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13742 Product name: Recombinant human DDRGK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-314 aa of BC000643 Sequence: MVAPVWYLVAAALLVGFILFLTRSRGRAASAGQEPLHNEELAGAGRVAQPGPLEPEEPRAGGRPRRRRDLGSRLQAQRRAQRVAWAEADENEEEAVILAQEEEGVEKPAETHLSGKIGAKKLRKLEEKQARKAQREAEEAEREERKRLESQREAEWKKEEERLRLEEEQKEEEERKAREEQAQREHEEYLKLKEAFVVEEEGVGETMTEEQSQSFLTEFINYIKQSKVVLLEDLASQVGLRTQDTINRIQDLLAEGTITGVIDDRGKFIYITPEELAAVANFIRQRGRVSIAELAQASNSLIAWGRESPAQAPA Predict reactive species |
| Full Name | DDRGK domain containing 1 |
| Calculated Molecular Weight | 314 aa, 36 kDa |
| Observed Molecular Weight | 36-46 kDa |
| GenBank Accession Number | BC000643 |
| Gene Symbol | DDRGK1 |
| Gene ID (NCBI) | 65992 |
| RRID | AB_2827383 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96HY6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DDRGK1, also name as C20orf116, is a member of the DDRGK domain-containing protein family with unknown function. Depletion of DDRGK1 inhibits cell proliferation and invasion. Expression of NF-κB target genes showed the most significant decrease after depleting of DDRGK1, suggesting that DDRGK1 may play an important role in the NF-κB signaling pathway. DDRGK1 interacts with IκBα and regulates its stability, thereby regulates the NF-κB transcriptional activity. DDRGK1 acts as an important regulator of the NF-κB pathway. (PMID: 23675531) Catalog#21445-1-AP is a rabbit polyclonal antibody raised against the full-length of human DDRGK1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DDRGK1 antibody 21445-1-AP | Download protocol |
| IHC protocol for DDRGK1 antibody 21445-1-AP | Download protocol |
| IP protocol for DDRGK1 antibody 21445-1-AP | Download protocol |
| WB protocol for DDRGK1 antibody 21445-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Cell A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation.
| ||
Nat Cell Biol UFMylation maintains tumour suppressor p53 stability by antagonizing its ubiquitination. | ||
Cell Res UFMylation of RPL26 links translocation-associated quality control to endoplasmic reticulum protein homeostasis. | ||
Mol Cell UFL1 ablation in T cells suppresses PD-1 UFMylation to enhance anti-tumor immunity
| ||
Adv Sci (Weinh) DDRGK1 Enhances Osteosarcoma Chemoresistance via Inhibiting KEAP1-Mediated NRF2 Ubiquitination
|
