Filter: AllWBIHCIF/ICC

WB analysis using 18323-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18323-1-AP (DEAF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 18323-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18323-1-AP (DEAF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 18323-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18323-1-AP (DEAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 18323-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18323-1-AP (DEAF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 18323-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18323-1-AP (DEAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 18323-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18323-1-AP (DEAF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 18323-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 18323-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 18323-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 18323-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin using 18323-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 18323-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human brain using 18323-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human brain using 18323-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human spleen using 18323-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human spleen using 18323-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung using 18323-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung using 18323-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 18323-1-AP (DEAF1 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of Jurkat using 18323-1-AP
Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using DEAF1 antibody (18323-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

DEAF1 Polyclonal antibody

Name: DEAF1 Polyclonal antibody
Brand: Proteintech
SKU: 18323-1-AP

DEAF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 18323-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ZMYND5, Zinc finger MYND domain-containing protein 5, Suppressin, SPN, NUDR

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, BxPC-3 cells, Jurkat cells, SH-SY5Y cells
Positive IHC detected in	human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human brain tissue, human spleen tissue, human lung tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18323-1-AP targets DEAF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13173 Product name: Recombinant human DEAF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-316 aa of BC053322 Sequence: MEDSDSAAKQLGLAEAAAVAAAAAVAAAAAAAAGGEAEEPVLSRDEDSEEDADSEAERETPRVTAVAVMAAEPGHMDMGAEALPGPDEAAAAAAFAEVTTVTVANVGAAADNVFTTSVANAASISGHVLSGRTALQIGDSLNTEKATLIVVHTDGSIVETTGLKGPAAPLTPGPQSPPTPLAPGQEKGGTKYNWDPSVYDSELPVRCRNISGTLYKNRLGSGGRGRCIKQGENWYSPTEFEAMAGRASSKDWKRSIRYAGRPLQCLIQDGILNPHAASCTCAACCDDMTLSGPVRLFVPYKRRKKENELPTTPVKK Predict reactive species
Full Name	deformed epidermal autoregulatory factor 1 (Drosophila)
Calculated Molecular Weight	565 aa, 59 kDa
Observed Molecular Weight	60-63 kDa, 72 kDa
GenBank Accession Number	BC053322
Gene Symbol	DEAF1
Gene ID (NCBI)	10522
RRID	AB_2261595
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75398
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for DEAF1 antibody 18323-1-AP	Download protocol
IHC protocol for DEAF1 antibody 18323-1-AP	Download protocol
WB protocol for DEAF1 antibody 18323-1-AP	Download protocol