DGCR8 Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB, IHC, IF, IP |
| Cited applications | IF, IHC, IP, RnaIP, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human, mouse |
| Positive WB detected in | Jurkat cells, HEK-293 cells, A431 cells, HeLa cells, mouse testis tissue |
| Positive IHC detected in | human breast cancer |
| Positive IF detected in | SH-SY5Y cells |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:50-1:200 IF: 1:20-1:100 |
IP = Immunoprecipitation
IP result of anti-DGCR8 (10996-1-AP for IP and Detection) with 293 cell.
Jurkat cells were subjected to SDS PAGE followed by western blot with 10996-1-AP(DGCR8 Antibody) at dilution of 1:800
Immunohistochemistry of paraffin-embedded human breast cancer using 10996-1-AP(DGCR8 Antibody) at Dilution 1:100 (under 10x lens)
Immunohistochemistry of paraffin-embedded human breast cancer using 10996-1-AP(DGCR8 Antibody) at Dilution 1:100 (under 40x lens)
Immunofluorescent analysis of SH-SY5Y cells, using DGCR8 antibody 10996-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | DGCR8 fusion protein ag1429 | Full name | DiGeorge syndrome critical region gene 8 |
|
Calculated molecular weight |
773aa,86kd |
Observed molecular
weight |
120kd |
|
GenBank accession number |
BC009323 | Gene ID (NCBI) | 54487 |
| Gene symbol | DGCR8 | Synonyms | C22orf12; DGCRK6; Gy1 |
DGCR8 is a RNA-binding protein that assists the Rnase III enzyme Drosha in the processing of microRNAs (miRNAs), which regulate the expression of a large number of protein-coding genes[PMID: 22580560]. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, may be an essential component of the primary miRNAs processing complex, along with Drosha, promoting the processing of primary microRNA to precursor microRNA. It is ubiquitous expressed in human and mouse tissues, and is deleted in DiGeorge syndrome[22323604].This antibody is a rabbit polyclonal antibody raised against residues near the C terminal DGCR8 of human origin. The antibody can recognize isoform1 and isoform2 of DGCR8. (NM_022720.6 (120kDa)& 001190326.1(86kDa), respective ) [PMID: 22086916] |
