Tested Applications
| Positive WB detected in | mouse brain tissue, human placenta tissue, THP-1 cells |
| Positive IHC detected in | human colon cancer tissue, human cervix tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse small intestine tissue, mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
Product Information
17885-1-AP targets DGKQ in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11938 Product name: Recombinant human DGKQ protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 595-942 aa of BC063801 Sequence: PKSGGLKGRDLLCSFRKLLNPHQVFDLTNGGPLPGLHLFSQVPCFRVLVCGGDGTVGWVLGALEETRYRLACPEPSVAILPLGTGNDLGRVLRWGAGYSGEDPFSVLLSVDEADAVLMDRWTILLDAHEAGSAENDTADAEPPKIVQMSNYCGIGIDAELSLDFHQAREEEPGKFTSRLHNKGVYVRVGLQKISHSRSLHKQIRLQVERQEVELPSIEGLIFINIPSWGSGADLWGSDSDTRFEKPRMDDGLLEVVGVTGVVHMGQVQGGLRSGIRIAQGSYFRVTLLKATPVQVDGEPWVQAPGHMIISAAGPKVHMLRKAKQKPRRAGTTRDARADAAPAPESDPR Predict reactive species |
| Full Name | diacylglycerol kinase, theta 110kDa |
| Calculated Molecular Weight | 942 aa, 101 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | BC063801 |
| Gene Symbol | DGKQ |
| Gene ID (NCBI) | 1609 |
| RRID | AB_2091305 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P52824 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Diacylglycerol kinase theta (DGKQ) is a mammalian enzyme that phosphorylates the lipid second messenger diacylglycerol (DAG) to produce phosphatidic acid (PA). This activity positions DGKQ as a central molecular switch, terminating DAG-mediated signaling (such as through protein kinase C) and initiating distinct PA-driven pathways. It is critically involved in diverse cellular processes, including the regulation of steroidogenic gene transcription in hormone signaling and synaptic vesicle endocytosis in neurons.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DGKQ antibody 17885-1-AP | Download protocol |
| IHC protocol for DGKQ antibody 17885-1-AP | Download protocol |
| WB protocol for DGKQ antibody 17885-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Med Rep Establishment of a novel hepatic steatosis cell model by Cas9/sgRNA-mediated DGKθ gene knockout.
| ||
J Sci Food Agric Neonatal resveratrol administration promotes skeletal muscle growth and insulin sensitivity in intrauterine growth-retarded suckling piglets associated with activation of FGF21-AMPKα pathway |
