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WB analysis of mouse kidney using 16314-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16314-1-AP (dGK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 16314-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16314-1-AP (dGK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human hepatocirrhosis using 16314-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 16314-1-AP (dGK antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human hepatocirrhosis using 16314-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 16314-1-AP (dGK antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human hepatocirrhosis using 16314-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16314-1-AP (dGK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human hepatocirrhosis using 16314-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16314-1-AP (dGK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human hepatocirrhosis using 16314-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16314-1-AP (dGK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human hepatocirrhosis using 16314-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16314-1-AP (dGK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

dGK Polyclonal antibody

Name: dGK Polyclonal antibody
Brand: Proteintech
SKU: 16314-1-AP

dGK Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16314-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

DGUOK, Deoxyadenosine kinase, mitochondrial, Deoxyguanosine kinase, EC:2.7.1.113, EC:2.7.1.76

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse kidney tissue
Positive IHC detected in	human hepatocirrhosis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16314-1-AP targets dGK in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9361 Product name: Recombinant human DGUOK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-277 aa of BC015757 Sequence: MAAGRLFLSRLRAPFSSMAKSPLEGVSSSRGLHAGRGPRRLSIEGNIAVGKSTFVKLLTKTYPEWHVATEPVATWQNIQAAGTQKACTAQSLGNLLDMMYREPARWSYTFQTFSFLSRLKVQLEPFPEKLLQARKPVQIFERSVYSDRYIFAKNLFENGSLSDIEWHIYQDWHSFLLWEFASRITLHGFIYLQASPQVCLKRLYQRAREEEKGIELAYLEQLHGQHEAWLIHKTTKLHFEALMNIPVLVLDVNDDFSEEVTKQEDLMREVNTFVKNL Predict reactive species
Full Name	deoxyguanosine kinase
Calculated Molecular Weight	277 aa, 32 kDa
Observed Molecular Weight	28-32 kDa, 56-64 kDa
GenBank Accession Number	BC015757
Gene Symbol	DGUOK
Gene ID (NCBI)	1716
RRID	AB_10638311
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16854
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Deoxyguanosine kinase (dGK) deficiency is a frequent cause of mitochondrial DNA depletion associated with a hepatocerebral phenotype. dGK is a member of the deoxyribonucleoside kinase family involved in phosphorylation of deoxyribonucleosides. In mammals, there are four deoxyribonucleoside kinases with overlapping specificities: two cytoplasmic enzymes, thymidine kinase 1 (TK1) and deoxycytidine kinase (dCK), and two mitochondrial enzymes, thymidine kinase 2 (TK2) and deoxyguanosine kinase (dGK). The human dGK protein is highly specific for purine substrates. Human dGK is a dimer of two identical subunits of 30 kDa. The three-dimensional structure of the human dGK monomer has been reported, providing an explanation for the substrate specificity of the enzyme. (PMID: 29786478, PMID: 11427893, PMID: 17073823)