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DHRS11 Polyclonal antibody

DHRS11 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20172-1-AP

Synonyms

ARPG836, DHRS11, SDR24C1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DHRS11 Polyclonal antibody (20172-1-AP)

    WB analysis using 20172-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20172-1-AP (DHRS11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using DHRS11 Polyclonal antibody (20172-1-AP)

    WB analysis of mouse liver using 20172-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 20172-1-AP (DHRS11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using DHRS11 Polyclonal antibody (20172-1-AP)

    WB analysis of COLO 320 using 20172-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 20172-1-AP (DHRS11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DHRS11 Polyclonal antibody (20172-1-AP)

    IHC staining of human breast cancer using 20172-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20172-1-AP (DHRS11 Antibody) at dilution of 1:50(under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DHRS11 Polyclonal antibody (20172-1-AP)

    IHC staining of human breast cancer using 20172-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20172-1-AP (DHRS11 Antibody) at dilution of 1:50(under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using DHRS11 Polyclonal antibody (20172-1-AP)

    IHC staining of mouse small intestine using 20172-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20172-1-AP (DHRS11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using DHRS11 Polyclonal antibody (20172-1-AP)

    IHC staining of mouse small intestine using 20172-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20172-1-AP (DHRS11 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse kidney tissue, COLO 320 cells, mouse liver tissue
Positive IHC detected inmouse small intestine tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20172-1-AP targets DHRS11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHRS11 fusion protein Ag14005
Full Name dehydrogenase/reductase (SDR family) member 11
Calculated Molecular Weight 260 aa, 28 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC002731
Gene Symbol DHRS11
Gene ID (NCBI) 79154
RRIDAB_10733521
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DHRS11 antibody 20172-1-APDownload protocol
IHC protocol for DHRS11 antibody 20172-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols