Tested Applications
| Positive WB detected in | SKOV-3 cells, human liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15735-1-AP targets DHRS2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8508 Product name: Recombinant human DHRS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-271 aa of BC007339 Sequence: QGWFHPCARLSVRMSSTGIDRKGVLANRVAVVTGSTSGIGFAIARRLARDGAHVVISSRKQQNVDRAMAKLQGEGLSVAGIVCHVGKAEDREQLVAKALEHCGGVDFLVCSAGVNPLVGSTLGTSEQIWDKILSVNVKSPALLLSQLLPYMENRRGAVILVSSIAAYNPVVALGVYNVSKTALLGLTRTLALELAPKDIRVNCVVPGIIKTDFSKVFHGNESLWKNFKEHHQLQRIGESEDCAGIVSFLCSPDASYVNGENIAVAGYSTRL Predict reactive species |
| Full Name | dehydrogenase/reductase (SDR family) member 2 |
| Calculated Molecular Weight | 300 aa, 32 kDa |
| Observed Molecular Weight | 27-30 kDa |
| GenBank Accession Number | BC007339 |
| Gene Symbol | DHRS2 |
| Gene ID (NCBI) | 10202 |
| RRID | AB_2091847 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13268 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DHRS2(Dehydrogenase/reductase SDR family member 2) is also named as dicarbonyl reductase HEP27, Protein D and belongs to the short-chain dehydrogenases/reductases (SDR) family. It displays NADPH-dependent dicarbonyl reductase activity in vitro with 3,4-Hexanedione, 2,3-Heptanedione and 1-Phenyl-1,2-propanedione as substrates and there is no reductase activity displayed in vitro with steroids, retinoids and sugars as substrates. This protein also may inhibit cell replication. It has 2 isoforms produced by alternative splicing with the molecular weight of 27 kDa and 29 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DHRS2 antibody 15735-1-AP | Download protocol |
| IP protocol for DHRS2 antibody 15735-1-AP | Download protocol |
| WB protocol for DHRS2 antibody 15735-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-DHRS2 antibody (Cat# 15735-1-AP) has 6 total citations published in Cell Death & Disease, Translational Oncology, Oncology Reports, Epigenetics, European Archives of Oto-Rhino-Laryngology, and Iranian Journal of Public Health. Research fields span lung tumorigenesis and DNA repair, ovarian cancer epigenetics, oxaliplatin resistance mechanisms, head and neck squamous cell carcinoma immune subtyping, and tumor diagnosis with prognostic value. Applications include WB, IHC, and RNAi validation in human samples.
| Species | Application | Title |
|---|---|---|
Transl Oncol STAMBPL1 promotes the progression of lung adenocarcinoma by inhibiting DHRS2 expression | ||
Epigenetics Decreased DHRS2 expression is associated with HDACi resistance and poor prognosis in ovarian cancer.
| ||
Eur Arch Otorhinolaryngol Single-cell immune subtyping of basophils in head and neck squamous cell carcinoma and a prognostic model based on bulk RNA sequencing. | ||
Iran J Public Health Auxiliary Diagnosis and Prognostic Value of Dehydrogenase/Reductase 2 (DHRS2) in Various Tumors |
