DIAPH3 Antibody
Rabbit Polyclonal
Catalog number: 14342-1-AP
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Applications
| Tested applications | ELISA, WB |
| Cited applications | WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human |
| Positive WB detected in | HeLa cells |
| Recommended dilution | WB: 1:500-1:5000 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
HeLa cells were subjected to SDS PAGE followed by western blot with 14342-1-AP(DIAPH3 Antibody) at dilution of 1:1000
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | DIAPH3 fusion protein ag5656 | Full name | diaphanous homolog 3 (Drosophila) |
|
Calculated molecular weight |
137kd |
Observed molecular
weight |
125-137kd |
|
GenBank accession number |
BC048963 | Gene ID (NCBI) | 81624 |
| Gene symbol | DIAPH3 | Synonyms | DKFZp434C0931; DKFZp686A13178; DRF3; FLJ34705; diap3; mDia2 |
Background
DIAPH3 is also refered as diaphanous-related formin-3 which binds to GTP-bound form of Rho and to profilin, acts in a Rho-dependent manner to recruit profilin to the membrane. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. Seven isoforms of DIAPH3 exist due to the alternative splicing. Recently it has been reported that dimerization and actin assembly activity of DIAPH3 isoforms 1 and 7 are essential for induction of specific cell protrusions. It may propose that isoform-selective actin assembly by DIAPH3 exerts specific and differentially regulated functions during cell adhesion and motility. |
