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WB analysis of mouse skeletal muscle using 21051-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21051-1-AP (DKK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 21051-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21051-1-AP (DKK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse skeletal muscle using 21051-1-AP
IP result of anti-DKK2 (IP:21051-1-AP, 4ug; Detection:21051-1-AP 1:300) with mouse skeletal muscle tissue lysate 4000ug.
IHC staining of human cervix using 21051-1-AP
Immunohistochemical analysis of paraffin-embedded human cervix using 21051-1-AP (DKK2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human cervix using 21051-1-AP
Immunohistochemical analysis of paraffin-embedded human cervix using 21051-1-AP (DKK2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin cancer using 21051-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21051-1-AP (DKK2 antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human heart using 21051-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 21051-1-AP (DKK2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 21051-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 21051-1-AP (DKK2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin cancer using 21051-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21051-1-AP (DKK2 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human skin cancer using 21051-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21051-1-AP (DKK2 antibody) at dilution of 1:200 (under 40x lens).

Featured Product
KD/KO Validated

DKK2 Polyclonal antibody

Name: DKK2 Polyclonal antibody
Brand: Proteintech
SKU: 21051-1-AP

DKK2 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 21051-1-AP

Publications(6)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Synonyms

Dickkopf 2, Dickkopf related protein 2, DKK 2, DKK2, hDkk 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information
Publications (6)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse skeletal muscle tissue
Positive IP detected in	mouse skeletal muscle tissue
Positive IHC detected in	human cervix tissue, human skin cancer tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IHC	See 2 publications below
IF	See 1 publications below

Product Information

21051-1-AP targets DKK2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12405 Product name: Recombinant human DKK2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-259 aa of BC075078 Sequence: MAALMRSKDSSCCLLLLAAVLMVESSQIGSSRAKLNSIKSSLGGETPGQAANRSAGMYQGLAFGGSKKGKNLGQAYPCSSDKECEVGRYCHSPHQGSSACMVCRRKKKRCHRDGMCCPSTRCNNGICIPVTESILTPHIPALDGTRHRDRNHGHYSNHDLGWQNLGRPHTKMSHIKGHEGDPCLRSSDCIEGFCCARHFWTKICKPVLHQGEVCTKQRKKGSHGLEIFQRCDCAKGLSCKVWKDATYSSKARLHVCQKI Predict reactive species
Full Name	dickkopf homolog 2 (Xenopus laevis)
Calculated Molecular Weight	259 aa, 28 kDa
Observed Molecular Weight	32 kDa
GenBank Accession Number	BC075078
Gene Symbol	DKK2
Gene ID (NCBI)	27123
RRID	AB_11232231
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UBU2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Mol Neurobiol Ionizing Radiation Blocks Hair Cell Regeneration in Zebrafish Lateral Line Neuromasts by Preventing Wnt Signaling. Authors - Rong Li View Article
mouse	WB	Int J Mol Sci Roles of Dkk2 in the Linkage from Muscle to Bone during Mechanical Unloading in Mice. Authors - Naoyuki Kawao View Article
human	IHC	Front Endocrinol (Lausanne) G Protein-Coupled Estrogen Receptor Correlates With Dkk2 Expression and Has Prognostic Impact in Ovarian Cancer Patients. Authors - Patricia Fraungruber View Article
mouse	WB	Exp Clin Endocrinol Diabetes Role of Dkk2 in the Muscle/bone Interaction of Androgen-Deficient Mice Authors - Shunki Iemura View Article
mouse	WB	Cell Death Discov Single-cell RNA-seq reveals cellular heterogeneity of mouse carotid artery under disturbed flow. Authors - Fengchan Li View Article
human	WB,IHC	J Immunol Res DKK2 Impairs Tumor Immunity Infiltration and Correlates with Poor Prognosis in Pancreatic Ductal Adenocarcinoma. Authors - Jianyu Yang KD Validated View Article