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KD/KO Validated

DLAT Polyclonal antibody

DLAT Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13426-1-AP

Publications (228)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, IP-MS, ELISA

Synonyms

Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, DLTA, EC:2.3.1.12, ODP2, PDC-E2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

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Filter: AllWBIPIHCIF/ICC

WB analysis using 13426-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13426-1-AP (DLAT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 13426-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13426-1-AP (DLAT antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 13426-1-AP
WB result of DLAT antibody (13426-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DLAT transfected HeLa cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HAP1 using 13426-1-AP
WB result of DLAT antibody (13426-1-AP; 1:60000; room temperature for 1.5 hours) with wild-type and DLAT knockout HAP1 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HepG2 using 13426-1-AP
IP result of anti-DLAT (IP:13426-1-AP, 3ug; Detection:13426-1-AP 1:1000) with HepG2 cells lysate 2000ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 13426-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 13426-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 13426-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 13426-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 13426-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13426-1-AP (DLAT antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 13426-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DLAT antibody (13426-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 13426-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLAT antibody (13426-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for DLAT in ?

Overview

Tested Applications
Product Information
Publications (228)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, A431 cells, HAP1 cells, HEK-293 cells, HepG2 cells, Jurkat cells, LNCaP cells, MCF-7 cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human liver cancer tissue, human colon cancer tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13426-1-AP targets DLAT in WB, IHC, IF/ICC, IP, CoIP, IP-MS, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, rabbit, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4226 Product name: Recombinant human DLAT protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-322 aa of BC039084 Sequence: MWRVCARRAQNVAPWAGLEARWTALQEVPGTPRVTSRSGPAPARRNSVTTGYGGVRALCGWTPSSGATPRNRLLLQLLGSPGRRYYSLPPHQKVPLPSLSPTMQAGTIARWEKKEGDKINEGDLIAEVETDKATVGFESLEECYMAKILVAEGTRDVPIGAIICITVGKPEDIEAFKNYTLDSSAAPTPQAAPAPTPAATASPPTPSAQAPGSSYPPHMQVLLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPLGTPLCIIVEKEADISAFADYRPTEVTDLKPQVPPPT Predict reactive species
Full Name	dihydrolipoamide S-acetyltransferase
Calculated Molecular Weight	647 aa, 70 kDa
Observed Molecular Weight	69-70 kDa
GenBank Accession Number	BC039084
Gene Symbol	DLAT
Gene ID (NCBI)	1737
RRID	AB_2091774
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P10515
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for DLAT antibody 13426-1-AP	Download protocol
IHC protocol for DLAT antibody 13426-1-AP	Download protocol
IP protocol for DLAT antibody 13426-1-AP	Download protocol
WB protocol for DLAT antibody 13426-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Anti-DLAT (Cat# 13426-1-AP) has accumulated 228 citations published in high-impact journals including Nature Cell Biology, Nature Communications, Advanced Materials, Advanced Science, Biomaterials, ACS Nano, Cell Death & Differentiation, and Free Radical Biology and Medicine. Associated research fields predominantly encompass cuproptosis mechanisms, cancer immunotherapy, nanomedicine-based therapeutics, mitochondrial biology, neurodegenerative diseases, cardiovascular pathology, and metabolic disorders.

Species	Application	Title
human	WB	Imeta Clinical features and molecular landscape of cuproptosis signature-related molecular subtype in gastric cancer Authors - Wei Chong View Article
mouse	WB	Cell Res Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation Authors - Yunzi Mao View Article
mouse	WB	Adv Mater One-Step Symbiosis of Bimetallic Peroxides Nanoparticles to Induce Ferroptosis/Cuproptosis and Activate cGAS-STING Pathway for Enhanced Tumor Immunotherapy Authors - Bin Liu View Article
human	IF	Adv Mater Combating Atherosclerosis with Chirality/Phase Dual-Engineered Nanozyme Featuring Microenvironment-Programmed Senolytic and Senomorphic Actions Authors - Chengjin Ding View Article
human, mouse	WB,IP,IF	Ann Rheum Dis Antimitochondrial antibodies in systemic sclerosis target enteric neurons and are associated with GI dysmotility. Authors - Zsuzsanna H McMahan View Article
human	WB,IF	Bioact Mater Regulating SLC7A11/GSH/GPX4 axis by glucose dyshomeostasis to simultaneously promote disulfidptosis, cuproptosis and ferroptosis. Authors - Mengsi Zhang View Article