Tested Applications
| Positive WB detected in | HEK-293 cells, human testis tissue, HepG2 cells, human liver tissue, HeLa cells, Jurkat cells, mouse kidney tissue, rat kidney tissue, mouse lung tissue, NIH/3T3 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11713-1-AP targets DNAJA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2284 Product name: Recombinant human DNAJA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-397 aa of BC008182 Sequence: MVKETTYYDVLGVKPNATQEELKKAYRKLALKYHPDKNPNEGEKFKQISQAYEVLSDAKKRELYDKGGEQAIKEGGAGGGFGSPMDIFDMFFGGGGRMQRERRGKNVVHQLSVTLEDLYNGATRKLALQKNVICDKCEGRGGKKGAVECCPNCRGTGMQIRIHQIGPGMVQQIQSVCMECQGHGERISPKDRCKSCNGRKIVREKKILEVHIDKGMKDGQKITFHGEGDQEPGLEPGDIIIVLDQKDHAVFTRRGEDLFMCMDIQLVEALCGFQKPISTLDNRTIVITSHPGQIVKHGDIKCVLNEGMPIYRRPYEKGRLIIEFKVNFPENGFLSPDKLSLLEKLLPERKEVEETDEMDQVELVDFDPNQERRRHYNGEAYEDDEHHPRGGVQCQTS Predict reactive species |
| Full Name | DnaJ (Hsp40) homolog, subfamily A, member 1 |
| Calculated Molecular Weight | 397 aa, 45 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC008182 |
| Gene Symbol | DNAJA1 |
| Gene ID (NCBI) | 3301 |
| RRID | AB_10637266 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P31689 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DNAJA1 antibody 11713-1-AP | Download protocol |
| IHC protocol for DNAJA1 antibody 11713-1-AP | Download protocol |
| WB protocol for DNAJA1 antibody 11713-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-DNAJA1 (Cat# 11713-1-AP) has 15 total citations spanning journals including Nature, Nature Cell Biology, Nature Communications, JCI, Cardiovascular Research, Molecular Therapy Nucleic Acids, Molecular Medicine Reports, Scientific Reports, Cancers, JBC, Cell Biochemistry and Function, Reproductive Toxicology, Molecular Biology of the Cell, FEBS Open Bio, and bioRxiv. Research fields cover mitochondrial UPR, protein phase separation, autophagy, cancer metastasis, thrombosis, blood-brain barrier integrity, chaperone-mediated proteostasis, spermatogenesis, and Golgi function.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Heat-shock chaperone HSPB1 regulates cytoplasmic TDP-43 phase separation and liquid-to-gel transition | ||
Nat Commun Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells. | ||
J Clin Invest Cul4A-DDB1-mediated monoubiquitination of phosphoglycerate dehydrogenase promotes colorectal cancer metastasis via increased S-adenosylmethionine.
| ||
Cardiovasc Res Hypoxia suppresses platelet activity through GPVI downregulation but paradoxically enhances arterial thrombosis via elevated vWF. | ||
Mol Ther Nucleic Acids Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction |
Reviews
What customers say
One reviewer (Filip K.) gave a 5-star rating for Western Blot at a 1:2000 dilution (lot 00041402). They recommended the antibody, noting that it specifically recognizes DNAJA1 and does not cross-react with DNAJA2, which they ran in parallel. Overall, the review highlights the antibody's strong specificity and reliable performance in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Filip (Verified Customer) (10-28-2024) | I recommend this antibody. It does not recognize DNAJA2, which we run in parallel to DNAJA1.
|
