Tested Applications
| Positive WB detected in | A375 cells, mouse kidney tissue, human brain tissue |
| Positive IP detected in | A375 cells |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12236-1-AP targets DNAJA2 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2877 Product name: Recombinant human DNAJA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-214 aa of BC015809 Sequence: MANVADTKLYDILGVPPGASENELKKAYRKLAKEYHPDKNPNAGDKFKEISFAYEVLSNPEKRELYDRYGEQGLREGSGGGGGMDDIFSHIFGGGLFGFMGNQSRSRNGRRRGEDMMHPLKVSLEDLYNGKTTKLQLSKNVLCSACSGQGGKSGAVQKCSACRGRGVRIMIRQLAPGMVQQMQSVCSDCNGEGEVINEKDRCKKCEGKKVIKEV Predict reactive species |
| Full Name | DnaJ (Hsp40) homolog, subfamily A, member 2 |
| Calculated Molecular Weight | 417 aa, 46 kDa |
| Observed Molecular Weight | 46 kDa |
| GenBank Accession Number | BC015809 |
| Gene Symbol | DNAJA2 |
| Gene ID (NCBI) | 10294 |
| RRID | AB_2230709 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60884 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DNAJA2, also named as CPR3, HIRIP4, and DNJ-14, belongs to the DNAJ/Hsp40 family of proteins. DNAJA2 is a key regulator of protein homeostasis that stimulates the ATPase activity of Hsp70 chaperones, thereby facilitating protein folding, assembly, translocation, and degradation. DNAJA2 has a molecular mass of approximately 45 kDa and contains a highly conserved J-domain essential for its interaction with Hsp70. It can be detected as a band around 45 kDa in various human cell lines.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DNAJA2 antibody 12236-1-AP | Download protocol |
| IP protocol for DNAJA2 antibody 12236-1-AP | Download protocol |
| WB protocol for DNAJA2 antibody 12236-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DNAJA2 Polyclonal antibody (Cat# 12236-1-AP) has 12 citations published in journals including Cancer Research, Nature Communications, Cell Discovery, Cell Reports, Journal of Biological Chemistry, Molecular Biology of the Cell, BMC Microbiology, International Journal of Cancer, Cancers, Virus Research, and bioRxiv. Research fields span protein homeostasis and chaperone biology, neurodegeneration (TDP-43), cancer (medulloblastoma, prostate cancer), viral infections (Japanese encephalitis, Newcastle disease), stress granules, and mitochondrial biology.
| Species | Application | Title |
|---|---|---|
Cancer Res N-myc-Mediated Translation Control Is a Therapeutic Vulnerability in Medulloblastoma | ||
Cell Discov Heat shock protein DNAJA2 regulates transcription-coupled repair by triggering CSB degradation via chaperone-mediated autophagy | ||
Cell Rep Human J-domain proteins promote stress granule disassembly and suppress neurodegeneration-linked protein aggregation. | ||
BMC Microbiol DNAJA2 inhibits Newcastle disease virus replication by targeting its V protein to modulate the MDA5-MAVS pathway. | ||
Int J Cancer Down-regulation of BiP/GRP78 sensitizes resistant prostate cancer cells to gene-therapeutic overexpression of REIC/Dkk-3. |
