Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IHC detected in | mouse testis tissue, human kidney tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17177-1-AP targets DNAJB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10878 Product name: Recombinant human DNAJB3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-145 aa of BC024013 Sequence: MVDYYEVLDVPRQASSEAIKKAYRKLALKWHPDKNPENKEEAERRFKQVAEAYEVLSDAKKRDIYDRYGEAGAEGGCTGGRPFEDPFEYVFSFRDPADVFREFFGGQDPFSFDLLGNPLENILGGSEELLGKQKQSVCTPFLCLQ Predict reactive species |
| Full Name | DnaJ (Hsp40) homolog, subfamily B, member 3 |
| Calculated Molecular Weight | 145 aa, 17 kDa |
| Observed Molecular Weight | 28-30 kDa |
| GenBank Accession Number | BC024013 |
| Gene Symbol | DNAJB3 |
| Gene ID (NCBI) | 414061 |
| RRID | AB_2094420 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WWF6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DNAJB3 antibody 17177-1-AP | Download protocol |
| WB protocol for DNAJB3 antibody 17177-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-DNAJB3 antibody, Catalog No. 17177-1-AP, has 8 total citations. These appear in Scientific Reports (×3), PLoS One (×2), Lipids in Health and Disease, International Journal of Molecular Sciences, and Genes (Basel). Associated research fields include ER stress, glucose uptake, insulin signaling, metabolic stress, obesity, heat shock protein expression, and DNAJB3 cochaperone function in human and mouse models.
| Species | Application | Title |
|---|---|---|
Lipids Health Dis Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue. | ||
Int J Mol Sci Expression of Human DNAJ (Heat Shock Protein-40) B3 in Humanized UDP-glucuronosyltransferase 1 Mice. | ||
Sci Rep DNAJB3 attenuates metabolic stress and promotes glucose uptake by eliciting Glut4 translocation.
| ||
Sci Rep Alpha lipoic acid attenuates ER stress and improves glucose uptake through DNAJB3 cochaperone.
| ||
Genes (Basel) Genetic Deletion of DNAJB3 Using CRISPR-Cas9, Produced Discordant Phenotypes
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