Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, Y79 cells, mouse brain tissue, mouse testis tissue, rat brain tissue, Jurkat cells, SH-SY5Y cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11707-1-AP targets DNAJB6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2258 Product name: Recombinant human DNAJB6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-241 aa of BC000177 Sequence: MVDYYEVLGVQRHASPEDIKKAYRKLALKWHPDKNPENKEEAERKFKQVAEAYEVLSDAKKRDIYDKYGKEGLNGGGGGGSHFDSPFEFGFTFRNPDDVFREFFGGRDPFSFDFFEDPFEDFFGNRRGPRGSRSRGTGSFFSAFSGFPSFGSGFSSFDTGFTSFGSLGHGGLTSFSSTSFGGSGMGNFKSISTSTKMVNGRKITTKRIVENGQERVEVEEDGQLKSLTINGKEQLLRLDNK Predict reactive species |
| Full Name | DnaJ (Hsp40) homolog, subfamily B, member 6 |
| Calculated Molecular Weight | 241 aa, 27 kDa |
| Observed Molecular Weight | 27 kDa, 38 kDa |
| GenBank Accession Number | BC000177 |
| Gene Symbol | DNAJB6 |
| Gene ID (NCBI) | 10049 |
| RRID | AB_2230757 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75190 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DNAJB6, belongs to the heat shock protein 40 (HSP40) family, a noncanonical member of the DNAJ-chaperone family, is mainly expressed in neurons. It plays an important role in recovery from misfolded protein aggregates (PMID: 27276715). As seen by the western blot stained for DNAJB6, there are 2 splice forms of the protein resulting in a longer isoform (38 kDa) and a shorter one (27 kDa). DNAJB6 (27 kDa) isoform but not DNAJB6 (38 kDa) isoform is effective with suppressing cytoplasmic protein aggregation (PMID:20159555).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DNAJB6 antibody 11707-1-AP | Download protocol |
| IHC protocol for DNAJB6 antibody 11707-1-AP | Download protocol |
| IP protocol for DNAJB6 antibody 11707-1-AP | Download protocol |
| WB protocol for DNAJB6 antibody 11707-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody is an anti-DNAJB6 antibody (Cat# 11707-1-AP), with a total of 13 citations. These appear in journals including Neurobiology of Disease, Journal of Parkinson's Disease, Glia, Cancers, iScience, Frontiers in Neuroscience, Journal of Proteome Research, Molecular Carcinogenesis, Journal of Molecular Histology, Biology of Methods and Protocols, STAR Protocols, eLife, and bioRxiv. Research fields span neurodegenerative diseases (Parkinson's, tau pathology), cancer (colorectal, cervical, hepatocellular), proteostasis and chaperone biology, and spermatogenesis.
| Species | Application | Title |
|---|---|---|
Neurobiol Dis DNAJB6 suppresses alpha-synuclein induced pathology in an animal model of Parkinson's disease. | ||
Cancers (Basel) Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin | ||
iScience Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis. | ||
Front Neurosci Detergent-insoluble PFN1 inoculation expedites disease onset and progression in PFN1 transgenic rats |
Reviews
What customers say
One reviewer (Elizabeth Hui) rated this DNAJB6 antibody 5 out of 5 stars. She used it for cell culture and protein purification with HEK293 cells and reported that it works well. Her lab's project focuses on DNAJB6 co-chaperone activity, and she stated they will continue to order this product. Overall, the feedback is positive, highlighting reliable performance for their research needs.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Elizabeth (Verified Customer) (01-19-2026) | The DNAJB6 antibody works well and we will continue to order this product. One of the project of our lab focuses on DNAJB6 co-chaperone activity.
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