Filter: AllWBIPIHC

WB analysis using 12362-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12362-1-AP (DNAJC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12362-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12362-1-AP (DNAJC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of Raji using 12362-1-AP
IP result of anti-DNAJC1 (IP:12362-1-AP, 4ug; Detection:12362-1-AP 1:1000) with Raji cells lysate 3200 ug.
IHC staining of human breast cancer using 12362-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12362-1-AP (DNAJC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 12362-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12362-1-AP (DNAJC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 12362-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12362-1-AP (DNAJC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 12362-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12362-1-AP (DNAJC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DNAJC1 Polyclonal antibody

Name: DNAJC1 Polyclonal antibody
Brand: Proteintech
SKU: 12362-1-AP

DNAJC1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 12362-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

DnaJ protein homolog MTJ1, DNAJC1, DNAJL1, ERdj1, HTJ1, MTJ1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	MCF-7 cells, A549 cells, Raji cells, NIH/3T3 cells
Positive IP detected in	Raji cells
Positive IHC detected in	human breast cancer tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

12362-1-AP targets DNAJC1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3030 Product name: Recombinant human DNAJC1 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 194-554 aa of BC030955 Sequence: TGSKSVDVSKLGASEKNERLLMKPQWHDLLPCKLGIWFCLTLKALPHLIQDAGQFYAKYKETRLKEKEDALTRTELETLQKQKKVKKPKPEFPVYTPLETTYIQSYDHGTSIEEIEEQMDDWLENRNRTQKKQAPEWTEEDLSQLTRSMVKFPGGTPGRWEKIAHELGRSVTDVTTKAKQLKDSVTCSPGMVRLSELKSTVQNSRPIKTATTLPDDMITQREDAEGVAAEEEQEGDSGEQETGATDARPRRRKPARLLEATAKPEPEEKSRAKRQKDFDIAEQNESSDEESLRKERARSAEEPWTQNQQKLLELALQQYPRGSSDCWDKIARCVPSKSKEDCIARYKLLVELVQKKKQAKS Predict reactive species
Full Name	DnaJ (Hsp40) homolog, subfamily C, member 1
Calculated Molecular Weight	554 aa, 64 kDa
Observed Molecular Weight	64 kDa
GenBank Accession Number	BC030955
Gene Symbol	DNAJC1
Gene ID (NCBI)	64215
RRID	AB_2094754
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96KC8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for DNAJC1 antibody 12362-1-AP	Download protocol
IP protocol for DNAJC1 antibody 12362-1-AP	Download protocol
WB protocol for DNAJC1 antibody 12362-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Cancer Res Clin Oncol DNAJC1 facilitates glioblastoma progression by promoting extracellular matrix reorganization and macrophage infiltration Authors - Han Zhang View Article