Tested Applications
| Positive WB detected in | mouse brain tissue, NIH/3T3 cells, A549 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | rat brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Neuro-2a cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18205-1-AP targets Dynamin 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig, chicken, fish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12827 Product name: Recombinant human DNM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 502-851 aa of BC063850 Sequence: RSNQMNKKKTSGNQDEILVIRKGWLTINNIGIMKGGSKEYWFVLTAENLSWYKDDEEKEKKYMLSVDNLKLRDVEKGFMSSKHIFALFNTEQRNVYKDYRQLELACETQEEVDSWKASFLRAGVYPERVGDKEKASETEENGSDSFMHSMDPQLERQVETIRNLVDSYMAIVNKTVRDLMPKTIMHLMINNTKEFIFSELLANLYSCGDQNTLMEESAEQAQRRDEMLRMYHALKEALSIIGDINTTTVSTPMPPPVDDSWLQVQSVPAGRRSPTSSPTPQRRAPAVPPARPGSRGPAPGPPPAGSALGGAPPVPSRPGASPDPFGPPPQVPSRPNRAPPGVPRITISDP Predict reactive species |
| Full Name | dynamin 1 |
| Calculated Molecular Weight | 851 aa, 96 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC063850 |
| Gene Symbol | DNM1 |
| Gene ID (NCBI) | 1759 |
| RRID | AB_2093197 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q05193 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Dynamin 1 antibody 18205-1-AP | Download protocol |
| IHC protocol for Dynamin 1 antibody 18205-1-AP | Download protocol |
| IP protocol for Dynamin 1 antibody 18205-1-AP | Download protocol |
| WB protocol for Dynamin 1 antibody 18205-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Dynamin 1 Polyclonal antibody (Cat# 18205-1-AP) has 10 citations published in journals including Neuron, PNAS, Neurobiology of Disease, Communications Biology, International Journal of Molecular Sciences, PLoS Pathogens, Journal of Neuroscience, Journal of Trace Elements in Medicine and Biology, Virus Research, and eLife. Research fields span neuroscience (synaptic dysfunction, sleep regulation, Parkinson's disease), oncology (lung cancer chemoresistance), virology (SARS-CoV-2, pseudorabies virus), mitochondrial biology, and lipid metabolism.
| Species | Application | Title |
|---|---|---|
Neuron Neurons undergo IFNγ-driven persistent epigenetic shifts and synaptopathy in encephalitis. | ||
Proc Natl Acad Sci U S A Calcineurin governs baseline and homeostatic regulations of non-rapid eye movement sleep in mice | ||
Neurobiol Dis Asparagine endopeptidase cleaves synaptojanin 1 and triggers synaptic dysfunction in Parkinson's disease. | ||
Commun Biol The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells
| ||
Int J Mol Sci Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish. | ||
PLoS Pathog Pseudorabies virus inhibits progesterone-induced inactivation of TRPML1 to facilitate viral entry |
