Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IP detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17191-1-AP targets DNMBP in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10973 Product name: Recombinant human DNMBP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 473-824 aa of BC041628 Sequence: RVLQQLQVFTFFPESLPATKKPFERKTIDRQSARKPLLGLPSYMLQSEELRASLLARYPPEKLFQAERNFNAAQDLDVSLLEGDLVGVIKKKDPMGSQNRWLIDNGVTKGFVYSSFLKPYNPRRSHSDASVGSHSSTESEHGSSSPRFPRQNSGSTLTFNPSSMAVSFTSGSCQKQPQDASPPPKEWDQGTLSASLNPSNSESSPSRCPSDPDSTSQPRSGDSADVARDVKQPTATPRSYRNFRHPEIVGYSVPGRNGQSQDLVKGCARTAQAPEDRSTEPDGSEAEGNQVYFAVYTFKARNPNELSVSANQKLKILEFKDVTGNTEWWLAEVNGKKGYVPSNYIRKTEYT Predict reactive species |
| Full Name | dynamin binding protein |
| Calculated Molecular Weight | 823aa,94 kDa; 1577aa,177 kDa |
| Observed Molecular Weight | 180 kDa |
| GenBank Accession Number | BC041628 |
| Gene Symbol | DNMBP |
| Gene ID (NCBI) | 23268 |
| RRID | AB_2230665 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6XZF7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for DNMBP antibody 17191-1-AP | Download protocol |
| WB protocol for DNMBP antibody 17191-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DNMBP Polyclonal antibody (Cat# 17191-1-AP) has 1 citation. It was used in a Western Blot study published in Aging (Albany NY) (2023), investigating the mechanism by which simultaneous intake of Jujuboside A and B regulates sleep at the hypothalamic level in mouse models. The research field encompasses neuroscience and pharmacology, specifically sleep regulation.
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) The mechanism of simultaneous intake of Jujuboside A and B in the regulation of sleep at the hypothalamic level |
