Tested Applications
| Positive WB detected in | HEK-293 cells, mouse brain tissue, mouse kidney tissue |
| Positive IF/ICC detected in | HepG2 cells, Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20574-1-AP targets DOC2B in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | double C2-like domains, beta |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 46 kDa |
| GenBank Accession Number | NM_003585 |
| Gene Symbol | DOC2B |
| Gene ID (NCBI) | 8447 |
| RRID | AB_10696316 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14184 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DOC2B may be involved in constitutive membrane trafficking including dynein-dependent intracellular vesicle transport. In vitro, binds calcium and phospholipids. The antibody is specific to DOC2B.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DOC2B antibody 20574-1-AP | Download protocol |
| WB protocol for DOC2B antibody 20574-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-DOC2B antibody (Cat# 20574-1-AP) has 9 citations published in journals including Science Advances, Pharmacological Research, Cell Communication and Signaling, Diabetes, Free Radical Biology and Medicine, Journal of Biological Chemistry, Scientific Reports, International Journal of Molecular Sciences, and Cell Biology and Toxicology. Research fields span cervical cancer biology, Wnt/β-catenin signaling, mitochondrial dysfunction, lipotoxicity, β-cell protection, epithelial-mesenchymal transition, glucose uptake regulation, and neurotransmitter release.
| Species | Application | Title |
|---|---|---|
Sci Adv Phosphorylation of Doc2 by EphB2 modulates Munc13-mediated SNARE complex assembly and neurotransmitter release | ||
Pharmacol Res DOC2B is a negative regulator of Wnt/β-catenin signaling pathway in cervical cancer.
| ||
Cell Commun Signal The ester derivative Palmitoylcarnitine abrogates cervical cancer cell survival by enhancing lipotoxicity and mitochondrial dysfunction | ||
Diabetes Doc2b Protects β-Cells Against Inflammatory Damage and Enhances Function.
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Free Radic Biol Med Double C-2 like domain beta (DOC2B) induces calcium dependent oxidative stress to promote lipotoxicity and mitochondrial dysfunction for its tumor suppressive function | ||
Cell Biol Toxicol Metastatic suppression by DOC2B is mediated by inhibition of epithelial-mesenchymal transition and induction of senescence.
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