Tested Applications
| Positive WB detected in | HEK-293 cells, LNCaP cells, MCF-7 cells, A431 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23421-1-AP targets DOCK180 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18069 Product name: Recombinant human DOCK1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1565-1865 aa of BC146857 Sequence: KDLIAWQIPFLAEGIRIHGDKVTEALRPFHERMEACFKQLKEKVEKEYGVRIMPSSLDDRRGSRPRSMVRSFTMPSSSRPLSVASVSSLSSDSTPSRPGSDGFALEPLLPKKMHSRSQDKLDKDDLEKEKKDKKKEKRNSKHQEIFEKEFKPTDISLQQSEAVILSETISPLRPQRPKSQVMNVIGSERRFSVSPSSPSSQQTPPPVTPRAKLSFSMQSSLELNGMTGADVADVPPPLPLKGSVADYGNLMENQDLLGSPTPPPPPPHQRHLPPPLPSKTPPPPPPKTTRKQTSVDSGIVQ Predict reactive species |
| Full Name | dedicator of cytokinesis 1 |
| Calculated Molecular Weight | 1865 aa, 215 kDa |
| Observed Molecular Weight | 215 kDa |
| GenBank Accession Number | BC146857 |
| Gene Symbol | DOCK1 |
| Gene ID (NCBI) | 1793 |
| RRID | AB_2879276 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14185 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DOCK180 antibody 23421-1-AP | Download protocol |
| IHC protocol for DOCK180 antibody 23421-1-AP | Download protocol |
| IP protocol for DOCK180 antibody 23421-1-AP | Download protocol |
| WB protocol for DOCK180 antibody 23421-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DOCK180 Polyclonal antibody (Cat# 23421-1-AP) has 8 citations published in journals including Biomaterials, Cell Death & Disease, Diabetes, Journal of Cell Biology, Experimental Cell Research, Acta Otolaryngologica, bioRxiv, and Advanced Healthcare Materials. Research fields span mechanotransduction, hepatocarcinoma, diabetic wound healing, Schwann cell myelination, colorectal cancer progression, auditory nerve development, and osteoarthritis repair. All cited applications are Western blot in human, mouse, or zebrafish samples.
| Species | Application | Title |
|---|---|---|
Biomaterials Stiffness assisted cell-matrix remodeling trigger 3D mechanotransduction regulatory programs | ||
Cell Death Dis Annexin A5 regulates hepatocarcinoma malignancy via CRKI/II-DOCK180-RAC1 integrin and MEK-ERK pathways. | ||
Diabetes Dedicator of Cytokinesis 5 Regulates Keratinocyte Function and Promotes Diabetic Wound Healing. | ||
J Cell Biol Dock1 functions in Schwann cells to regulate development, maintenance, and repair
| ||
Exp Cell Res Acetylation of ELMO1 correlates with Rac1 activity and colorectal cancer progress | ||
Acta Otolaryngol C1QL1 regulates auditory nerve fibers growth via ELMO1-DOCK180-RAC1 integrin |
Reviews
What customers say
One reviewer (K. Pang) rated this antibody 5 stars for Western Blot on human liver cells. They used a 1:500 dilution with 5% BSA in TBST as blocking buffer, incubating at 4°C overnight. The review was posted in October 2023 for lot 00019025. Overall, the single review reflects a positive experience with straightforward WB protocol conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
k. (Verified Customer) (10-26-2023) | I used it for WB with 5% BSA+TBST at 4°C overnight.
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