Tested Applications
| Positive WB detected in | mouse testis tissue, SH-SY5Y cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 4 publications below |
| IF | See 2 publications below |
Product Information
11015-2-AP targets DOM3Z in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1491 Product name: Recombinant human DOM3Z protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-396 aa of BC009344 Sequence: MDPRGTKRGAEKTEVAEPRNKLPRPAPSLPTDPALYSGPFPFYRRPSELGCFSLDAQRQYHGDARALRYYSPPPTNGPGPNFDLRDGYPDRYQPRDEEVQERLDHLLCWLLEHRGRLEGGPGWLAEAIVTWRGHLTKLLTTPYERQEGWQLAASRFQGTLYLSEVETPNARAQRLARPPLLRELMYMGYKFEQYMCADKPGSSPDPSGEVNTNVAFCSVLRSRLGSHPLLFSGEVDCTDPQAPSTQPPTCYVELKTSKEMHSPGQWRSFYRHKLLKWWAQSFLPGVPNVVAGFRNPDGFVSSLKTFPTMKMFEYVRNDRDGWNPSVCMNFCAAFLSFAQSTVVQDDPRLVHLFSWEPGGPVTVSVHQDAPYAFLPIWYVEAMTQDLPSPPKTPSPK Predict reactive species |
| Full Name | dom-3 homolog Z (C. elegans) |
| Calculated Molecular Weight | 43.6 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC009344 |
| Gene Symbol | DOM3Z |
| Gene ID (NCBI) | 1797 |
| RRID | AB_2095257 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O77932 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DOM3Z also known as DXO, NG6, is a 396 amino acid ubiquitously expressed protein. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6 and possesses RNA 5'-pyrophosphohydrolase activity. Chromosome 6 contains 170 million base pairs and represents nearly 6% of the human genome. Partial deletion of the q arm of chromosome 6 is associated with early-onset colorectal cancer. DOM3Z is ubiquitously expressed, with high expression in the testis (PMID: 9799600).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DOM3Z antibody 11015-2-AP | Download protocol |
| IHC protocol for DOM3Z antibody 11015-2-AP | Download protocol |
| IP protocol for DOM3Z antibody 11015-2-AP | Download protocol |
| WB protocol for DOM3Z antibody 11015-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell 5' End Nicotinamide Adenine Dinucleotide Cap in Human Cells Promotes RNA Decay through DXO-Mediated deNADding.
| ||
Mol Cell A mammalian pre-mRNA 5' end capping quality control mechanism and an unexpected link of capping to pre-mRNA processing.
| ||
RNA Unraveling regulation and new components of human P-bodies through a protein interaction framework and experimental validation. | ||
PLoS One 2'-O-methylation of the mRNA cap protects RNAs from decapping and degradation by DXO. | ||
Nat Chem Biol Structural and mechanistic basis of mammalian Nudt12 RNA deNADding.
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