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WB analysis of HepG2 using 11568-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11568-1-AP (DPP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of A2780 using 11568-1-AP
A2780 cells were subjected to SDS PAGE followed by western blot with 11568-1-AP (DPP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 11568-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 11568-1-AP (DPP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 11568-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 11568-1-AP (DPP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 11568-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 11568-1-AP (DPP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human ovary tumor using 11568-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11568-1-AP (DPP9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 11568-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11568-1-AP (DPP9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 11568-1-AP
Immunofluorescent analysis of HepG2 cells using 11568-1-AP (DPP9 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of MCF-7 using 11568-1-AP
Immunofluorescent analysis of MCF-7 cells using 11568-1-AP (DPP9 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of PC-3 using 11568-1-AP
Immunofluorescent analysis of PC-3 cells using 11568-1-AP (DPP9 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

DPP9 Polyclonal antibody

Name: DPP9 Polyclonal antibody
Brand: Proteintech
SKU: 11568-1-AP

DPP9 Polyclonal Antibody for WB, IF, IHC, ELISA

Cat No. 11568-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Synonyms

dipeptidyl peptidase 9, Dipeptidyl peptidase IX, DP9, DPLP9, DPP IX, DPP9, DPRP 2, DPRP2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells, A2780 cells, MCF-7 cells, mouse heart tissue, PC-3 cells
Positive IHC detected in	human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, MCF-7 cells, PC-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11568-1-AP targets DPP9 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2145 Product name: Recombinant human DPP9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 514-863 aa of BC037948 Sequence: YFQGTKDTPLEHHLYVVSYEAAGEIVRLTTPGFSHSCSMSQNFDMFVSHYSSVSTPPCVHVYKLSGPDDDPLHKQPRFWASMMEAASCPPDYVPPEIFHFHTRSDVRLYGMIYKPHALQPGKKHPTVLFVYGGPQVQLVNNSFKGIKYLRLNTLASLGYAVVVIDGRGSCQRGLRFEGALKNQMGQVEIEDQVEGLQFVAEKYGFIDLSRVAIHGWSYGGFLSLMGLIHKPQVFKVAIAGAPVTVWMAYDTGYTERYMDVPENNQHGYEAGSVALHVEKLPNEPNRLLILHGFLDENVHFFHTNFLVSQLIRAGKPYQLQIYPNERHSIRCPESGEHYEVTLLHFLQEYL Predict reactive species
Full Name	dipeptidyl-peptidase 9
Calculated Molecular Weight	863 aa, 98 kDa
Observed Molecular Weight	98 kDa
GenBank Accession Number	BC037948
Gene Symbol	DPP9
Gene ID (NCBI)	91039
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q86TI2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dipeptidyl peptidase 9(DPP9), is a member of the S9B family in clan SC of the serine proteases. DPP9 has 3 isoforms with MW of 95, 98 and 101 kDa. DPP9 is involved in the regulation of the activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer.