Tested Applications
| Positive WB detected in | PC-12 cells, mouse brain tissue, rat brain tissue, SH-SY5Y cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14686-1-AP targets CRMP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5985 Product name: Recombinant human DPYSL2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 223-572 aa of BC056408 Sequence: HVLSRPEEVEAEAVNRAITIANQTNCPLYITKVMSKSSAEVIAQARKKGTVVYGEPITASLGTDGSHYWSKNWAKAAAFVTSPPLSPDPTTPDFLNSLLSCGDLQVTGSAHCTFNTAQKAVGKDNFTLIPEGTNGTEERMSVIWDKAVVTGKMDENQFVAVTSTNAAKVFNLYPRKGRIAVGSDADLVIWDPDSVKTISAKTHNSSLEYNIFEGMECRGSPLVVISQGKIVLEDGTLHVTEGSGRYIPRKPFPDFVYKRIKARSRLAELRGVPRGLYDGPVCEVSVTPKTVTPASSAKTSPAKQQAPPVRNLHQSGFSLSGAQIDDNIPRRTTQRIVAPPGGRANITSLG Predict reactive species |
| Full Name | dihydropyrimidinase-like 2 |
| Calculated Molecular Weight | 62 kDa |
| Observed Molecular Weight | 62-66 kDa |
| GenBank Accession Number | BC056408 |
| Gene Symbol | CRMP2 |
| Gene ID (NCBI) | 1808 |
| RRID | AB_10638925 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16555 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Dihydropyrimidinase-like 2 (Dpysl2), a protein involved in neuronal differentiation and axonal guidance is found altered in various nervous system diseases (PMID:19524114). It is also named as CRMP2, ULIP2 and belongs to hydantoinase/dihydropyrimidinase subfamily. The phosphorylation of the particular portion carboxyl-terminal to the basic region of DPYSL2 may play an important role in regulating its activity, and may be involved in the formation of degenerating neurites in AD brain(PMID:10757975).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CRMP2 antibody 14686-1-AP | Download protocol |
| IHC protocol for CRMP2 antibody 14686-1-AP | Download protocol |
| WB protocol for CRMP2 antibody 14686-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Cancer Res Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification. | ||
Biochim Biophys Acta Mol Basis Dis Anti-CRMP2 antibody induces anxiety-like behavior and increases pyramidal neuron excitability in mice | ||
Nat Commun A branching model of lineage differentiation underpinning the neurogenic potential of enteric glia | ||
Eur J Med Res Screening and identification of differentially expressed miRNA and mRNA for intervertebral disc degeneration on bioinformatics. | ||
Neural Regen Res Adeno-associated virus-mediated inhibition of ROCK2 promotes synaptogenesis and neurogenesis in rats after ischemic stroke. | ||
CNS Neurosci Ther Autoantibodies Against Collapsin Response Mediator Proteins Associated With Encephalopathy/Myelopathy: A Single-Center Retrospective Study. |
